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ZIRC
ZFIN ID: ZDB-FIG-140211-45
Bräutigam et al., 2013 - Glutaredoxin regulates vascular development by reversible glutathionylation of sirtuin 1. Proc. Natl. Acad. Sci. USA   110(50):20057-20062 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 1

zfGrx2 is essential for development of the vasculature. (A) Confocal microscopy of fli:EGFP transgenic embryos with or without zfGrx2 at 35 and 48 h postfertilization (hpf). DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; PC, parachordal sprouts. (Scale bars, 100 μm.) (B) Statistical analysis of A shows mean ± SEM, significance (***P < 0.01) was calculated by two-tailed Student’s t test, 50 pg WT or mutated zfGrx2 mRNA were injected per single egg for rescue experiments. (C) Statistical analysis of intersegmental vessel formation in fli:EGFP embryos, zfGrx2 knockdown embryos, and zfGrx2 knockdown embryos rescued with 15 ng/embryo zfSIRT1 mRNA (48 hpf) (Fig. S2), mean ± SEM, significance (*P < 0.05) was calculated by two-tailed Student’s t test. (D) Quantitative real-time PCR of zfSirt1 transcripts in WT and zfGrx2 knockdown embryos 48 hpf. Presented are mean ± SEM of triplicates of two independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 posterior cardinal vein IFL
Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec posterior cardinal vein IFL
y1Tg + MO1-glrx2 standard conditions Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 posterior cardinal vein IFL
Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec posterior cardinal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-glrx2 standard conditions Prim-15 intersegmental vessel malformed, abnormal
Prim-15 trunk vasculature malformed, abnormal
Prim-15 vasculature development process quality, abnormal
Long-pec intersegmental vessel malformed, abnormal
Long-pec trunk vasculature malformed, abnormal
Long-pec vasculature development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA