ZFIN ID: ZDB-FIG-140130-29
Kochhan et al., 2013 - Blood Flow Changes Coincide with Cellular Rearrangements during Blood Vessel Pruning in Zebrafish Embryos. PLoS One   8(10):e75060 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to High-pec

Fig. 2

Endothelial cells rearrange from a multicellular into a partially unicellular tube during blood vessel pruning.

(A) Still images from a time-lapse movie of Tg(kdrl:cytobow1.0)mu125; Tg(HSP:Cre)zdf13 embryos. Imaged area indicated with box in Figure 1D. Bracket marks dorsal CrDI. At the 37.5 hpf time point, note unicellular connection between the dorsal CrDI and the NCA (single green cell, marked with red bracket). (B) Merged still images from time-lapse imaging of Tg(UAS:RFP); Tg(fliep:Gal4FF)ubs4; Tg(UAS:VE-cadherin-deltaC-EGFP)ubs12 between 36 and 45 hpf. Red bracket at 42.5 hpf indicates unicellular connection between dorsal CrDI and NCA. (C) Still images from Tg(UAS:VE-cadherin-deltaC-EGFP)ubs12 expression corresponding to images in B. (D) Schematic drawing of cell rearrangements shown in B, C. Brackets label dorsal CrDI, purple arrow highlights NCA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkuasrfp1aTg; ubs12Tg; ubs4Tg standard conditions Prim-25 to High-pec cranial division of the internal carotid artery cell junction IFL
Prim-25 to High-pec nasal ciliary artery cell junction IFL
RFP nkuasrfp1aTg; ubs12Tg; ubs4Tg standard conditions Prim-25 to High-pec cranial division of the internal carotid artery IFL
Prim-25 to High-pec nasal ciliary artery IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One