ZFIN ID: ZDB-FIG-140114-32
Wilkins et al., 2013 - p53-Mediated Biliary Defects Caused by Knockdown of cirh1a, the Zebrafish Homolog of the Gene Responsible for North American Indian Childhood Cirrhosis. PLoS One   8(10):e77670 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 5

Cirhin-deficient larvae have defects in biliary and canalicular morphology.

(A-C) Confocal projections of 5 dpf livers immunostained with antibodies against canalicular marker MDR (red) and biliary epitope 2F11 (green). Livers from fish with normal BODIPY-FL C16 processing (A) show elongated canaliculi intersecting with normally arborized biliary networks. In contrast, fish with reduced fluorescent lipid processing (B and C) show truncated, rounded canalicular profiles and more sparse biliary networks. Insets show high-magnification view of one or few cells to highlight canalicular morphology. (D) Semiquantitative scoring of canalicular morphology, with 1+=0-25% elongated, 2+=26-50% elongated, 3+=51-75% elongated, and 4+=76-100% elongated. *, p<0.05 vs Mismatch MO 1 ng.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-2F11 IHC WT control Day 5 bile ductule
IHC WT + MO1-utp4 standard conditions Day 5 bile ductule
IHC WT + MO2-utp4 standard conditions Day 5 bile ductule
Ab1-mdr IHC WT control Day 5 bile canaliculus
IHC WT + MO1-utp4 standard conditions Day 5 bile canaliculus
IHC WT + MO2-utp4 standard conditions Day 5 bile canaliculus
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-utp4 standard conditions Day 5 bile canaliculus decreased branchiness, abnormal
WT + MO1-utp4 standard conditions Day 5 bile canaliculus decreased length, abnormal
WT + MO1-utp4 standard conditions Day 5 bile canaliculus spheroid, abnormal
WT + MO1-utp4 standard conditions Day 5 bile ductule sparse, abnormal
WT + MO2-utp4 standard conditions Day 5 bile canaliculus decreased branchiness, abnormal
Day 5 bile canaliculus decreased length, abnormal
Day 5 bile canaliculus spheroid, abnormal
Day 5 bile ductule sparse, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One