ZFIN ID: ZDB-FIG-140114-26
Corallo et al., 2013 - Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals. Development (Cambridge, England)   140(22):4594-4601 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2 Hh signaling is upregulated in Emilin3 morphant embryos. (A) Wild-type (WT) and Tg12x_Gli embryos were treated with 100 μM cyclopamine (cyc) and probed at 24 hpf by in situ hybridization for ptc1 or mCherry. (B) qRT-PCR for ptc1 and mCherry transcripts in Emilin3 morphant and control embryos (*P<0.05; n=30). (C) In situ hybridization for nkx2.2a, tbx20 and myoD1 in 24 hpf injected embryos. All images are lateral views, except for bottom panels (dorsal views). (D) In situ hybridization for shh, ehh and ntl in 24 hpf injected embryos. (E) Immunofluorescence for engrailed in 24 hpf injected embryos. Scale bars: 150 μm. (F) Quantification of engrailed-positive cells at 24 hpf (*P<0.05; n=10). Data are mean+s.e.m. Ctrl MO, control morpholino; e3a MO, emilin3a morpholino; e3b MO, emilin3b morpholino; e3a+e3b MO, emilin3a + emilin3b morpholinos; MPs, muscle pioneers; MFFs, medial fast fibers.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ihhb WT control Prim-5 notochord distal region ISH
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 notochord distal region ISH
myod1 WT control Prim-5 trunk ISH
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 trunk ISH
nkx2.2a WT control Prim-5 trunk ISH
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 trunk ISH
ptch1 WT control Prim-5 trunk ISH
WT chemical treatment: Cyclopamine Prim-5 Not Detected trunk ISH
WT + MO2-emilin3a + MO2-emilin3b chemical treatment: Cyclopamine Prim-5 Not Detected trunk ISH
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 trunk ISH
ia10Tg control Prim-5 trunk ISH
ia10Tg + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 trunk ISH
shha WT control Prim-5 notochord distal region ISH
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 notochord distal region ISH
tbx20 WT control Prim-5 trunk ISH
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 trunk ISH
tbxta WT control Prim-5 notochord distal region ISH
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 notochord distal region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC WT control Prim-5 notochord
IHC WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 notochord
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-emilin3a + MO1-emilin3b standard conditions Prim-5 post-vent region muscle pioneer increased amount, abnormal
Prim-5 trunk fast muscle cell increased amount, abnormal
Prim-5 trunk muscle pioneer increased amount, abnormal
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 post-vent region muscle pioneer increased amount, abnormal
Prim-5 smoothened signaling pathway increased process quality, abnormal
Prim-5 trunk fast muscle cell increased amount, abnormal
Prim-5 trunk muscle pioneer increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development