FIGURE

Fig. 1

ID

ZDB-FIG-140114-25

Publication

Corallo et al., 2013 - Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals

Other Figures

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Fig. 1

Emilin3 is an essential component of the notochord sheath. (A) Lateral views of zebrafish embryos after immunofluorescence for Emilin3 and collagen II. Scale bars: 30 μm. (B) Lateral views of 24 hpf injected embryos. (C) BODIPY TR Ceramide staining of notochord cells in 24 hpf injected embryos. Scale bar: 50 μm. (D) Quantification of the mean number of vacuolated cells within the trunk region of control and treated notochord (not significantly different, n=10). Data are mean+s.e.m. (E) Transmission electron micrographs of the notochord sheath in transverse trunk sections of 30 hpf injected embryos. The arrow points at the medial layer of the notochord basement membrane, which is thinner and partially disorganized in Emilin3 double morphants. Scale bar: 500 nm. (F) Immunofluorescence for Emilin3 and collagen II in 24 hpf injected embryos. Scale bar: 10 μm. (G) Lateral views of 24 hpf injected embryos. col2a1 MO, col2a1 morpholino; Ctrl MO, control morpholino; e3a MO, emilin3a morpholino; e3b MO, emilin3b morpholino; i, inner layer; m, medial layer; no, notochord; o, outer layer.

Expression Data

Antibodies:	Ab1-col2a Ab1-emilin3
Fish:	WT WT + MO2-emilin3a + MO2-emilin3b
Knockdown Reagents:	MO2-emilin3a MO2-emilin3b
Anatomical Terms:	notochord notochord anatomical surface
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-col2a1a WT + MO2-emilin3a WT + MO2-emilin3b WT + MO2-emilin3a + MO2-emilin3b WT + MO2-col2a1a + MO2-emilin3a + MO2-emilin3b
Knockdown Reagents:	MO2-col2a1a MO2-emilin3a MO2-emilin3b
Observed In:	notochord notochord extracellular matrix notochord inner cell somite whole organism
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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