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ZFIN ID: ZDB-FIG-140114-25
Corallo et al., 2013 - Emilin3 is required for notochord sheath integrity and interacts with Scube2 to regulate notochord-derived Hedgehog signals. Development (Cambridge, England)   140(22):4594-4601 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 1 Emilin3 is an essential component of the notochord sheath. (A) Lateral views of zebrafish embryos after immunofluorescence for Emilin3 and collagen II. Scale bars: 30 μm. (B) Lateral views of 24 hpf injected embryos. (C) BODIPY TR Ceramide staining of notochord cells in 24 hpf injected embryos. Scale bar: 50 μm. (D) Quantification of the mean number of vacuolated cells within the trunk region of control and treated notochord (not significantly different, n=10). Data are mean+s.e.m. (E) Transmission electron micrographs of the notochord sheath in transverse trunk sections of 30 hpf injected embryos. The arrow points at the medial layer of the notochord basement membrane, which is thinner and partially disorganized in Emilin3 double morphants. Scale bar: 500 nm. (F) Immunofluorescence for Emilin3 and collagen II in 24 hpf injected embryos. Scale bar: 10 μm. (G) Lateral views of 24 hpf injected embryos. col2a1 MO, col2a1 morpholino; Ctrl MO, control morpholino; e3a MO, emilin3a morpholino; e3b MO, emilin3b morpholino; i, inner layer; m, medial layer; no, notochord; o, outer layer.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-col2a IHC WT control Prim-5 notochord
IHC WT standard conditions 20-25 somites notochord
IHC Prim-5 notochord
IHC Long-pec notochord
IHC WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 notochord
Ab1-emilin3 IHC WT control Prim-5 notochord anatomical surface
IHC WT standard conditions 20-25 somites notochord
IHC Prim-5 notochord anatomical surface
IHC Long-pec Not Detected notochord
IHC WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 Not Detected notochord
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-col2a1a standard conditions Prim-5 whole organism curved ventral, abnormal
WT + MO2-col2a1a + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 notochord deformed, abnormal
Prim-5 somite shape, abnormal
Prim-5 whole organism curved ventral, abnormal
WT + MO2-emilin3a standard conditions Prim-5 notochord morphology, normal
Prim-15 notochord extracellular matrix morphology, normal
WT + MO2-emilin3a + MO2-emilin3b standard conditions Prim-5 notochord deformed, abnormal
Prim-5 notochord inner cell normal amount, normal
Prim-5 somite shape, abnormal
Prim-15 notochord extracellular matrix decreased thickness, abnormal
Prim-15 notochord extracellular matrix disorganized, abnormal
WT + MO2-emilin3b standard conditions Prim-5 notochord morphology, normal
Prim-15 notochord extracellular matrix morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development