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ZFIN ID: ZDB-FIG-131108-3
Zaucker et al., 2013 - notch3 is essential for oligodendrocyte development and vascular integrity in zebrafish. Disease models & mechanisms   6(5):1246-59 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 2

Transient reduction in OPCs in notch3 mutants at stages at which vessel patterning is intact. (A–C) olig2 at 50 hpf was similar in (A,A2) wild type and (B,B2) notch3st51/+ heterozygotes, but was reduced in OPCs (compare red dashed areas) of (C,C2) notch3st51/st51 mutants. Arrow indicates region devoid of olig2-expressing cells. (D–F) sox10 expression at 52 hpf was comparable in (D) wild type and (E) notch3st51/+ heterozygotes, but was reduced in the midline of the hindbrain (compare yellow dashed areas) in (F) notch3st51/st51 mutants. (A–C) Lateral views. (A2–C2,D–I) Dorsal views. (G–I) Vessel pattern at the anatomical level is intact in notch3zm/zm mutants. Max projection images of Tg[fli1a:EGFP] labeled vasculature of (G) wild type (n=9), (H) notch3zm/+ heterozygotes (n=9) and (I) notch3zm/zm mutants (n=5). CtAs, central arteries; PHBC, primordial hindbrain channel; BA, basilar artery; MCeV, mid-cerebral vein; PCS, posterior connecting segment. The orientation of the anterior (A) and posterior (P) axis is indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP notch3zm197263Tg/+; y1Tg standard conditions Long-pec basilar artery IFL
Long-pec central artery IFL
Long-pec cranial vasculature IFL
Long-pec mid cerebral vein IFL
Long-pec primordial hindbrain channel IFL
notch3zm197263Tg/zm197263Tg; y1Tg standard conditions Long-pec basilar artery IFL
Long-pec central artery IFL
Long-pec cranial vasculature IFL
Long-pec mid cerebral vein IFL
Long-pec primordial hindbrain channel IFL
y1Tg standard conditions Long-pec basilar artery IFL
Long-pec central artery IFL
Long-pec cranial vasculature IFL
Long-pec mid cerebral vein IFL
Long-pec primordial hindbrain channel IFL
olig2 WT standard conditions Long-pec oligodendrocyte ISH
notch3st51/+ standard conditions Long-pec oligodendrocyte ISH
notch3st51/st51 standard conditions Long-pec oligodendrocyte ISH
sox10 WT standard conditions Long-pec oligodendrocyte ISH
notch3st51/+ standard conditions Long-pec oligodendrocyte ISH
notch3st51/st51 standard conditions Long-pec oligodendrocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
notch3st51/st51 standard conditions Long-pec oligodendrocyte development arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.