ZFIN ID: ZDB-FIG-131009-11
Hisano et al., 2013 - Functional cooperation of spns2 and fibronectin in cardiac and lower jaw development. Biology Open   2(8):789-794 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. S1

Knockdown phenotype of S1PR2 in fn mutant. (A,B) Cardiac morphology visualized by mRFP expression derived from Tg(cmlc2:mRFP). Both images show ventral views at 28 hpf. (C,D) Lower jaw morphology at 4 dpf was visualized by Alcian Blue staining (ventral view). Genotyping was performed by genomic sequencing after taking pictures. wt (A,C) and fn mutant (B,D). Scale bars: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mRFP fn1akt259/kt259; ko07Tg + MO1-s1pr2 standard conditions Prim-5 heart tube IFL
ko07Tg + MO1-s1pr2 standard conditions Prim-5 heart tube IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-s1pr2 standard conditions Day 4 pharyngeal arch 1 decreased length, abnormal
Day 4 pharyngeal arch 1 morphology, abnormal
fn1akt259/kt259 + MO1-s1pr2 standard conditions Day 4 pharyngeal arch 1 decreased length, abnormal
Day 4 pharyngeal arch 1 morphology, abnormal
fn1akt259/kt259; ko07Tg + MO1-s1pr2 standard conditions Prim-5 cardioblast migration to the midline involved in heart rudiment formation absent, abnormal
Prim-5 cardioblast migration to the midline involved in heart rudiment formation disrupted, abnormal
Prim-5 heart tube increased amount, abnormal
Prim-5 heart tube mislocalised laterally, abnormal
ko07Tg + MO1-s1pr2 standard conditions Prim-5 cardioblast migration to the midline involved in heart rudiment formation decreased process quality, abnormal
Prim-5 cardioblast migration to the midline involved in heart rudiment formation disrupted, abnormal
Prim-5 heart tube increased amount, abnormal
Prim-5 heart tube mislocalised laterally, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open