ZFIN ID: ZDB-FIG-130826-29
Gabor et al., 2013 - Super Resolution Microscopy Reveals that Caveolin-1 Is Required for Spatial Organization of CRFB1 and Subsequent Antiviral Signaling in Zebrafish. PLoS One   8(7):e68759 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2

Cav-1b expression is modulated during virus infection, and Cav-1b knockdown leaves morphants susceptible to infection.

A) Quantitative RT-PCR results revealed fold changes in the expression levels of Cav-1b in infected embryos when compared to uninfected embryos. Zebrafish were exposed seven dpf to 1×106 TCID50/mL virus. Total RNA was isolated from at 12, 24, and 48 hours post infection and reverse transcribed to cDNA (n = 20 fish per time point). All expression values have been normalized to the zebrafish β-actin gene. Error bars represent SEM of three replicates. B) Zebrafish embryos that were injected with Cav-1b morpholino (MO) to knock down the expression of Cav-1b or control MO were infected 48 hpf with 1×106 TCID50/ml virus and monitored for mortality. Results are representative of three separate experiments. Statistical analysis (Wilcoxon test) of the Kaplan-Meier curve was performed (*, p = 0.008). C) Zebrafish embryos that were injected with Cav-1b MO to knock down the expression of Cav-1b or control MO were infected by static immersion 48 hpf with 1×106 TCID50/ml virus. The graph indicates that early in infection (0–12 hpi), there is no difference in viral burden between Cav-1b morphants and controls. However, by 24–48 hpi, Cav-1b morphants have a higher viral burden. Figure is representative of three experiments; error bars are standard error of the mean (*, p<0.05). D) Western blot showing efficacy of MO knockdown in zebrafish. Zebrafish embryos from Control and Cav-1b MO, and Control MO with SHRV infection were compared for cav-1b expression at the 72 hpf developmental stage. At this time, infected fish were 24 hpi. Membranes were re-probed with antibody against β-actin to control for protein loading.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT viral treatment: Snakehead virus Prim-5 whole organism decreased life span, abnormal
WT + MO2-cav1 standard conditions Protruding-mouth whole organism decreased life span, abnormal
WT + MO2-cav1 viral treatment: Snakehead virus Prim-5 whole organism decreased life span, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One