ZFIN ID: ZDB-FIG-130820-581
Li et al., 2013 - Gpr125 modulates Dishevelled distribution and planar cell polarity signaling. Development (Cambridge, England)   140(14):3028-3039 Full text @ Development
Anatomical Terms:
Stage Range: 4-cell to Day 5

Fig. S1 Predicted protein domains and spatiotemporal expression profile of gpr125 during early zebrafish development. (A) Schematics of predicted zebrafish Gpr125 protein domains. The percentage amino acid identity or similarity between Gpr125 peptide sequences in vertebrates for the whole protein are included in parentheses, and for individual domains on the schema. LRR_RI, leucine-rich repeat_ribonuclease inhibitor type; IG_like, immunoglobulin_like; HRM, hormone receptor domain; GPS, GPCR proteolytic site; 7TM_2, seven-pass transmembrane type 2; ICD, intracellular domain. The PDZ binding motif (ETTV) at the C-terminus are labeled in pink. (B) RT-PCR of gpr125 transcripts from 1 to 120 hpf. b-Actin was used as a loading control. (C-F) Whole-mount in situ hybridization profile of gpr125 expression. Lateral views, anterior is upwards in C,D and leftwards in E,F. fb, forebrain; hb, hindbrain; mb, midbrain; mhb, midbrain-hindbrain boundary; ov, otic vesicle; pa, pharyngeal arches; pf, pectoral fin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
adgra3 WT standard conditions 8-cell whole organism ISH
4-cell to Day 5 whole organism RTPCR
75%-epiboly whole organism ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
Prim-5 otic vesicle ISH
Long-pec midbrain hindbrain boundary ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development