ZFIN ID: ZDB-FIG-130820-580
Li et al., 2013 - Gpr125 modulates Dishevelled distribution and planar cell polarity signaling. Development (Cambridge, England)   140(14):3028-3039 Full text @ Development
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. S2 Effective gpr125 MOs cause no noticeable morphological defects in wild-type embryos. (A-D) Live images of uninjected (A), 100 pg 5′-gpr125-T1-GFP RNA (GFP reporter for MO1-gpr125) injected (B), or 100 pg 5′-gpr125-T1-GFP RNA and MO1- gpr125 co-injected embryos (C,D) at the 70%-epiboly stage. (E-H) Live images of uninjected (E), 100 pg 5′-gpr125-T2-GFP RNA (GFP reporter for MO2-gpr125) injected (F), or 100 pg 5′-gpr125-T2-GFP RNA and MO2-gpr125 co-injected embryos (G-H) at the 75% epiboly stage. (I) Western blot quantification of GFP reporter protein levels in MO1-gpr125 injected embryos. The density of the GFP bands was normalized to that of the actin bands and the signal intensity relative to uninjected embryos is shown. Error bars indicate s.e.m. **P<0.01, ***P<0.001. (J,K) Lateral views of 5.4 ng MO1-gpr125-injected wild-type (J) or p53M214K/M214K embryos (K) at 1 dpf. Anterior is leftwards. Arrows in J indicate regions of significant cell death. (L-O) Whole-mount in situ hybridization analyses of marker gene expression in uninjected (L,N) and 2.7 ng MO1-gpr125-injected embryos (M,O) at the two-somite stage. (L,M) Animal pole views, ventral is upwards. (N,O) Dorsal views, anterior is upwards. n, notochord; ne, neural ectoderm border; pm, prechordal mesoderm; s, somites. Red line in N indicates the width of the notochord at the first somites. (P-W) Uninjected embryos or embryos injected with increasing doses of MO2-gpr125 at 1 dpf or 3 dpf.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-adgra3 standard conditions Prim-5 embryonic morphogenesis process quality, normal
Protruding-mouth embryonic morphogenesis process quality, normal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development