ZFIN ID: ZDB-FIG-130710-10
Rodríguez-Aznar et al., 2013 - Scratch2 Prevents Cell Cycle Re-Entry by Repressing miR-25 in Postmitotic Primary Neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience   33(12):5095-5105 Full text @ J. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP knu3Tg + MO1-mir25 + MO2-scrt2 + MO4-tp53 standard conditions Prim-5 spinal cord neuron IHC
knu3Tg + MO2-scrt2 standard conditions Prim-5 spinal cord neuron IHC
isl2a WT control Prim-5 Rohon-Beard neuron ISH
Prim-5 spinal cord primary motor neuron ISH
WT + MO1-mir25 + MO2-scrt2 + MO4-tp53 standard conditions Prim-5 Rohon-Beard neuron ISH
Prim-5 spinal cord primary motor neuron ISH
WT + MO1-mir25 + MO4-tp53 standard conditions Prim-5 Rohon-Beard neuron ISH
Prim-5 spinal cord primary motor neuron ISH
WT + MO2-scrt2 standard conditions Prim-5 Rohon-Beard neuron ISH
Prim-5 spinal cord primary motor neuron ISH
mcm7 WT control Prim-5 whole organism RTPCR
WT + MO2-scrt2 standard conditions Prim-5 whole organism RTPCR
mir25 WT control Prim-5 whole organism RTPCR
WT + MO2-scrt2 standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT control Prim-5 spinal cord neuron
IHC WT + MO1-mir25 + MO2-scrt2 + MO4-tp53 standard conditions Prim-5 spinal cord neuron
IHC WT + MO1-mir25 + MO4-tp53 standard conditions Prim-5 spinal cord neuron
IHC WT + MO2-scrt2 standard conditions Prim-5 spinal cord neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mir25 standard conditions Prim-5 apoptotic process increased occurrence, abnormal
Prim-5 central nervous system apoptotic, abnormal
WT + MO1-mir25 + MO2-scrt2 + MO4-tp53 standard conditions Prim-5 re-entry into mitotic cell cycle occurrence, normal
Prim-5 spinal cord has normal numbers of parts of type primary motor neuron, normal
WT + MO1-mir25 + MO4-tp53 standard conditions Prim-5 apoptotic process occurrence, normal
Prim-5 re-entry into mitotic cell cycle occurrence, normal
Prim-5 spinal cord has normal numbers of parts of type primary motor neuron, normal
WT + MO2-scrt2 standard conditions Prim-5 primary neuron proliferative, abnormal
Prim-5 proliferative region mislocalised, abnormal
Prim-5 re-entry into mitotic cell cycle increased occurrence, abnormal
Prim-5 regulation of neural precursor cell proliferation decreased process quality, abnormal
Prim-5 spinal cord has extra parts of type primary motor neuron, abnormal
knu3Tg + MO1-mir25 + MO2-scrt2 + MO4-tp53 standard conditions Prim-5 re-entry into mitotic cell cycle occurrence, normal
knu3Tg + MO2-scrt2 standard conditions Prim-5 primary neuron proliferative, abnormal
Prim-5 proliferative region mislocalised, abnormal
Prim-5 re-entry into mitotic cell cycle increased occurrence, abnormal
Prim-5 regulation of neural precursor cell proliferation decreased process quality, abnormal
Full text @ J. Neurosci.
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