header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-130607-7
Matsuda et al., 2013 - Lef1 regulates Dusp6 to influence neuromast formation and spacing in the zebrafish posterior lateral line primordium. Development (Cambridge, England)   140(11):2387-2397 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 1 Expression of rspo3 and lef1 is determined by similar mechanisms but knockdown of rspo3 and of lef1 have distinct effects on the pattern of neuromast deposition. (A-I) The leading end of the PLLp and caudal end of the zebrafish embryo are to the right. (A-F) The PLLp is outlined (dotted line). (A-D) Expression of rspo3 in the PLLp at 31 hpf. (A) rspo3 is expressed in the leading domain of the wild-type (WT) PLLp. (B) Heat shock-induced expression of a dominant-repressor form of TCF (ΔNTcf) inhibits rspo3 expression. (C,D) Exaggeration of Wnt activity, following inhibition of FGFR signaling with SU5402 (C) or in apc mutants (D), expands rspo3 expression toward the trailing domain. (E,F) rspo3 expression in the lef1 morphant PLLp (E) and lef1 expression in the rspo3 morphant PLLp (F) at 31 hpf. (G-K) The pattern of neuromast deposition in lef1 morphants and rspo3 morphants. (G-I) Neuromast deposition in tg[cldnb:lynGFP] embryos injected with lef1-MO, rspo3-MO or control-MO (conMO) at 48 hpf. White arrows indicate individual neuromasts and yellow arrowheads indicate PLLp termination. (J,K) Average position of each deposited neuromast in lef1 or rspo3 morphants relative to the distance from the otic vesicle (ov) to the tip of the tail. (H,J) Neuromasts are spaced further apart in rspo3 morphants. See supplementary material Fig. S4 for suppression of this phenotype by rspo3 mRNA co-injection. (I,K) Neuromasts are spaced closer together in lef1 morphants. Error bars indicate s.d.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP zf106Tg control Long-pec neuromast IFL
zf106Tg + MO3-lef1 + MO4-tp53 standard conditions Long-pec neuromast IFL
zf106Tg + MO3-rspo3 + MO4-tp53 standard conditions Long-pec neuromast IFL
lef1 WT + MO3-rspo3 + MO4-tp53 standard conditions Prim-15 posterior lateral line primordium ISH
rspo3 WT standard conditions Prim-15 posterior lateral line primordium ISH
WT chemical treatment: SU5402 Prim-15 posterior lateral line primordium ISH
WT + MO3-lef1 + MO4-tp53 standard conditions Prim-15 posterior lateral line primordium ISH
apchu745/hu745 standard conditions Prim-15 posterior lateral line primordium ISH
w26Tg heat shock Prim-15 Not Detected posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf106Tg + MO3-lef1 + MO4-tp53 standard conditions Long-pec neuromast decreased distance neuromast, abnormal
Long-pec neuromast deposition process quality, abnormal
zf106Tg + MO3-rspo3 + MO4-tp53 standard conditions Long-pec neuromast increased distance neuromast, abnormal
Long-pec neuromast deposition process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development