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ZIRC
ZFIN ID: ZDB-FIG-130509-4
Dabir et al., 2013 - A small molecule inhibitor of redox-regulated protein translocation into mitochondria. Developmental Cell   25(1):81-92 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 7 Embryos (3 hpf) were treated with 2.5 μM MitoBloCK-6 (B, E, and H) or 1% DMSO (A, D, and G) or embryos were injected with an ATG morpholino against ALR (C and F). Development was visualized by microscopy at 72 hpf (A–C). Erythrocytes were visualized by o-dianisidine staining at 72 hpf (D–F); arrows indicate regions of red blood cell accumulation in wild-type fish. Fluorescence microscopy of zebrafish hearts (72 hpf) that contained a mitochondrial-targeted DsRed included embryos treated with 1% DMSO (G), 2.5 μM MitoBloCK-6 (H), and buffer only (I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed la3002Tg control Protruding-mouth heart mitochondrion IFL
la3002Tg chemical treatment: pharmaceutical Protruding-mouth heart mitochondrion IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la3002Tg chemical treatment: pharmaceutical Protruding-mouth heart malformed, abnormal
Protruding-mouth heart looping process quality, abnormal
slc45a2b4(TL, TU) chemical treatment: pharmaceutical Protruding-mouth caudal fin lacks parts or has fewer parts of type nucleate erythrocyte, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth nucleate erythrocyte accumulation ball, abnormal
Protruding-mouth whole organism curved ventral, abnormal
slc45a2b4 + MO4-gfer(TL, TU) standard conditions Protruding-mouth caudal fin lacks parts or has fewer parts of type nucleate erythrocyte, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth nucleate erythrocyte accumulation ball, abnormal
Protruding-mouth whole organism curved ventral, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 25(1), Dabir, D.V., Hasson, S.A., Setoguchi, K., Johnson, M.E., Wongkongkathep, P., Douglas, C.J., Zimmerman, J., Damoiseaux, R., Teitell, M.A., and Koehler, C.M., A small molecule inhibitor of redox-regulated protein translocation into mitochondria, 81-92, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell