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ZFIN ID: ZDB-FIG-130429-15
Liu et al., 2013 - Direct and indirect roles of Fgf3 and Fgf10 in innervation and vascularisation of the vertebrate hypothalamic neurohypophysis. Development (Cambridge, England)   140(5):1111-1122 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Day 5

Fig. 6 NH innervation and vascularisation are compromised in zebrafish fgf3 mutants. Whole-mount in situ hybridisation (A-H), or immunohistochemical analyses (whole-mounts: I,J,M,N; sections: K,L,O,P), and confocal images (Q-T) of wild-type (wt) and fgf3–/– zebrafish; lateral (A-P) or ventral (Q-T) views; anterior to the left. (A-H) fgf3–/– display robust crabp1a expression in the NH anlage at 33 hpf (B) and 48 hpf (D); prl+ AH cells are largely (B) or completely (D) lost. At 72 hpf, crapb1a expression is moderately reduced (E,F); only a remnant of diffuse expression detected at 120 hpf (G,H). (I-L) Axons enter the NH and then extend throughout its antero-posterior length in wt larvae, but remain outside the fgf3 mutant NH. AH and NH outlined by yellow dots. (M-P) At 120 hpf, AcTub+ axons in the wt NH include both TH+ (M) and Avpl+ (O) axons. Neither is detected in fgf3 mutant NH (N,P). (Q-T) fgf3 mutants display normal numbers of Avpl+ neurons in the hypothalamus (Q,R), but lose Avpl+ axonal NH innervation (T), the hypophyseal artery and the two bilateral hypophyseal capillaries (T). The hypophyseal vein remains present (T). Q and R display maximal confocal projections through midbrain area. Arrow (Q) points to pituitary region, magnified and intensified in S. Panels S and T show single confocal sections at higher magnification in same specimens as in Q and R. Red arrows (S) point to hypophyseal capillaries; green arrowheads to GFP+ axons of avpl:egfp+ neurons projecting to NH; green arrow to GFP+ axonal termini within NH. ah, adenohypophysis; cadi, caudal division of internal carotid artery; hyv, hypophyseal vein; nihl, nucleus of the inferior hypothalamic lobe; nh, neurohypophysis; oc, oral cavity; po, preoptic area. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
avp Ab2-avp WT standard conditions Day 5 neurohypophysis axon terminus IHC
Ab2-avp fgf3t24152/t24152 standard conditions Day 5 Not Detected neurohypophysis axon terminus IHC
crabp1a WT standard conditions Prim-15 neurohypophysis ISH
Long-pec neurohypophysis ISH
Protruding-mouth neurohypophysis ISH
Day 5 neurohypophysis ISH
fgf3t24152/t24152 standard conditions Prim-15 neurohypophysis ISH
Long-pec neurohypophysis ISH
Protruding-mouth neurohypophysis ISH
Day 5 neurohypophysis ISH
mCherry fgf3t24152/t24152; s896Tg standard conditions Day 5 Not Detected hypophyseal artery blood vessel endothelial cell IHC
Day 5 Not Detected hypophyseal capillary blood vessel endothelial cell IHC
Day 5 hypophyseal vein blood vessel endothelial cell IHC
s896Tg standard conditions Day 5 hypophyseal artery blood vessel endothelial cell IHC
Day 5 hypophyseal capillary blood vessel endothelial cell IHC
Day 5 hypophyseal vein blood vessel endothelial cell IHC
prl WT standard conditions Prim-15 adenohypophyseal placode ISH
Long-pec adenohypophysis IHC
Long-pec adenohypophysis ISH
Protruding-mouth prolactin secreting cell ISH
Day 5 prolactin secreting cell ISH
Day 5 prolactin secreting cell IHC
fgf3t24152/t24152 standard conditions Prim-15 adenohypophyseal placode ISH
Long-pec Not Detected adenohypophysis IHC
Long-pec Not Detected adenohypophysis ISH
Protruding-mouth Not Detected adenohypophysis ISH
Day 5 Not Detected adenohypophysis IHC
Day 5 Not Detected adenohypophysis ISH
th WT standard conditions Day 5 neurohypophysis axon terminus IHC
fgf3t24152/t24152 standard conditions Day 5 Not Detected neurohypophysis axon terminus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-tuba IHC WT standard conditions Long-pec neurohypophysis axon terminus
IHC Day 5 neurohypophysis axon terminus
IHC fgf3t24152/t24152 standard conditions Long-pec Not Detected neurohypophysis axon terminus
IHC Day 5 Not Detected neurohypophysis axon terminus
Phenotype Details
Fish Conditions Stage Phenotype
fgf3t24152/t24152 standard conditions Prim-15 adenohypophysis decreased object quality, abnormal
Prim-15 adenohypophysis formation decreased process quality, abnormal
Long-pec adenohypophysis decreased object quality, abnormal
Long-pec adenohypophysis formation decreased process quality, abnormal
Protruding-mouth adenohypophysis decreased object quality, abnormal
Protruding-mouth adenohypophysis formation decreased process quality, abnormal
Day 5 adenohypophysis decreased object quality, abnormal
Day 5 adenohypophysis formation decreased process quality, abnormal
Day 5 neurohypophysis innervation decreased occurrence, abnormal
fgf3t24152/t24152; s896Tg standard conditions Day 5 adenohypophysis decreased object quality, abnormal
fgf3t24152/t24152; s896Tg standard conditions Day 5 adenohypophysis formation decreased process quality, abnormal
fgf3t24152/t24152; s896Tg standard conditions Day 5 blood vasculature lacks all parts of type hypophyseal artery, abnormal
fgf3t24152/t24152; s896Tg standard conditions Day 5 blood vasculature lacks all parts of type hypophyseal capillary, abnormal
fgf3t24152/t24152; s896Tg standard conditions Day 5 hypophyseal vein present, normal
fgf3t24152/t24152; s896Tg standard conditions Day 5 neurohypophysis vasculogenesis decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development