FIGURE

Fig. 5

ID

ZDB-FIG-130404-9

Publication

Law et al., 2013 - Maternal pak4 Expression is Required for Primitive Myelopoiesis in Zebrafish

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Fig. 5

Regulatory level of pak4 function. Flat mounts of in situ hybridization for ETS class transcription factors fli1a (column A) and fli1b (column B), etsrp1 (column C) and ets1 (column D), and the VEGF receptor flk1 (column E) reveal different dependence on pak4. Expression of the ETS transcription factors was not inhibited at any stage tested, however, expression of fli1b was transiently elevated at in the posterior domain, and etsrp1 in the rostral domain at the 5-somite stage (B and C; red arrowheads). flk1 Expression was reduced in both the anterior and posterior hematopoietic domains (E; red arrowheads). Frequency data are tabulated in (F).

Expression Data

Genes:	ets1 etsrp fli1rs kdrl
Fish:	EKW EKW + MO3-pak4 + MO4-pak4 + MO5-pak4
Knockdown Reagents:	MO3-pak4 MO4-pak4 MO5-pak4
Anatomical Term:	hematopoietic system
Stage Range:	5-9 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 130(2-3), Law, S.H., and Sargent, T.D., Maternal pak4 Expression is Required for Primitive Myelopoiesis in Zebrafish, 181-194, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.