FIGURE

Fig. 5

ID
ZDB-FIG-130326-66
Publication
Zhang et al., 2013 - The Role of egr1 in Early Zebrafish Retinogenesis
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Immunohistochemical analysis of the GCs in the Egr1-morphant retinas.

Immunohistochemical analysis of the GCs in the controls (5misCTLMO) and Egr1 morphants (egr1sMO) was performed by anti-zn8 (zn8; green) at 72 hpf (A & B) and 120 hpf (C & D). Phalloidin (red) was used as a counterstain to highlight the plexiform layers. A whole-eye section is shown at the top for each condition, while the magnified view of a selected region (white box) on the dorsal side of the optic nerve is shown at the bottom. The analysis has indicated that Egr1 knockdown suppressed the early dendritic outgrowth of GCs into the IPL at 72hpf (B), which was irregular at this stage. In addition, the cell number per retinal area was not different between the two groups. This defect was largely resolved by 120 hpf, despite the IPL was still thinner as shown in Figure 3. This suggests that there were still defects in differentiation of cells that projected neurites into the IPL. One possible cause of the defect is the differentiation problem of ACs as shown in Figure 4. See text, Table 1 and 2 for further discussion. For the whole-eye sections, the lens is on the left and dorsal is up. Scale bar = 50 µm for the whole-eye sections and 25 µm for the selected regions.

Expression Data
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One