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ZFIN ID: ZDB-FIG-130326-49
Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression. BMC Developmental Biology   12(1):37 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15

Fig. 6 Up-regulation and nuclear localization of pax4 and arx altered transcript. (AD) Confocal images of the pancreas area of 30 hpf embryos co-stained for pax4 mRNA (green) and Topro3 (blue) to visualize all cell nuclei, in control morphants (A-C) and pax4 morphants (B-D). Scale bar = 20 μm. (E-H) Confocal images of the pancreas area of 30 hpf embryos analyzed by fluorescent WISH with arx probe (red) and stained for Topro3 (blue). arx expression in somites and the floor plate of control morphants (E, G) and of arx morphants (F, H). Scale bar = 20 μm. (I-L) Ventral view of WISH 30 hpf embryos showing upregulation of arx transcripts in somites and floor plate in Moarx (J) and MoTarx (L) morphants. Original magnification 200x. (M – P) Ventral views of the pancreas area from embryos analyzed by WISH showing expression of pax4 in control morphants (M), pax4 morphants (N), arx morphants (O) and double pax4/arx morphants (P). Original magnification 400x. Anterior part of all the embryos is in the left. Asterisks indicate arx positive cells. fp: floor plate, s: somite, cont: control morphant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
arxa WT control Prim-15 floor plate ISH
Prim-15 pancreatic bud ISH
Prim-15 somite ISH
WT + MO1-pax4 + MO3-arxa standard conditions Prim-15 floor plate ISH
Prim-15 pancreatic bud ISH
Prim-15 somite ISH
WT + MO3-arxa standard conditions Prim-15 floor plate ISH
Prim-15 pancreatic bud ISH
Prim-15 somite ISH
WT + MO4-arxa standard conditions Prim-15 floor plate ISH
Prim-15 pancreatic bud ISH
Prim-15 somite ISH
pax4 WT control Prim-15 pancreatic bud ISH
WT + MO1-pax4 standard conditions Prim-15 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.