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ZFIN ID: ZDB-FIG-130326-47
Djiotsa et al., 2012 - Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression. BMC Developmental Biology   12(1):37 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 4 arx is required for the differentiation of α-cells. (A - H) Ventral views of the pancreas area from embryos analyzed by WISH, anterior to the left. Pancreatic expression of insulin (A, B), somatostatin (C, D), ghrelin (E, F) and glucagon (G, H) in control morphants (A, C, E, F) and arx morphants (B, D, F, G) at 30n hpf. Quantifications (I - K) represent the number of positive cells per embryo for control morphants and arx morphants. Original magnification 400x.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gcga WT control Prim-15 ghrelin secreting cell ISH
Prim-15 glucagon secreting cell ISH
Prim-15 pancreatic A cell ISH
Prim-15 pancreatic epsilon cell ISH
WT + MO3-arxa standard conditions Prim-15 Not Detected glucagon secreting cell ISH
Prim-15 Not Detected pancreatic A cell ISH
ghrl WT control Prim-15 ghrelin secreting cell ISH
Prim-15 pancreatic epsilon cell ISH
WT + MO3-arxa standard conditions Prim-15 ghrelin secreting cell ISH
Prim-15 pancreatic epsilon cell ISH
ins WT control Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
WT + MO3-arxa standard conditions Prim-15 insulin secreting cell ISH
Prim-15 pancreatic B cell ISH
sst2 WT control Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
WT + MO3-arxa standard conditions Prim-15 D cell ISH
Prim-15 somatostatin secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-arxa standard conditions Prim-15 glucagon secreting cell decreased amount, abnormal
Prim-15 pancreatic A cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.