ZFIN ID: ZDB-FIG-130318-29
Qian et al., 2013 - ENC1-like Integrates the Retinoic Acid/FGF Signaling Pathways to Modulate Ciliogenesis of Kupffer's Vesicle during Zebrafish Embryonic Development. Developmental Biology   374(1):85-95 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage: Bud

Fig. 4 Enc1l expression is controlled by RA, FGF, and Wnt signaling in the KV of zebrafish embryos. The embryos were stained at the bud stage. (A): In control embryos, enc1l is expressed in the KV. (B, C) In fgfr1-MO injected or SU5402-treated embryos, the expression of enc1l in KV is reduced compared to control. (D): The enc1l expression level in KV is increased by over-expression of fgf8-mRNA. (E): The enc1l expression level is reduced in RA-treated embryos. (F): The enc1l expression level is increased by DEAB treatment. (G): Injection of wnt8-MO reduces enc1l expression in the KV. (H): Treatment with LiCl, which increases canonical Wnt signaling, increases enc1l expression level. (I): Overexpression of wnt8-mRNA increases enc1l expression. (J): Injection of ntl-MO can reduce the enc1l expression level. Up- (L) and down- (K) regulation of FGF signaling has no effect on RA-treated embryos; however, in DEAB-treated embryos, the down-regulation of FGF signaling (M) can restore the enc1l expression level, though the up-regulation of FGF signaling (N) has no affect. In DEAB-treated embryos, injection of wnt8-MO (O) or wnt8-mRNA (P) restores enc1l expression. The number of the representative phenotype embryos versus the number of total analyzed embryosis shown at the top of the right-hand corner of the picture.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
enc3 WT standard conditions Bud forerunner cell group ISH
Bud germ ring ISH
WT chemical treatment: 4-(diethylamino)benzaldehyde Bud forerunner cell group ISH
Bud germ ring ISH
WT chemical treatment: pharmaceutical Bud forerunner cell group ISH
Bud germ ring ISH
WT chemical treatment: all-trans-retinoic acid Bud forerunner cell group ISH
Bud germ ring ISH
WT chemical treatment: SU5402 Bud forerunner cell group ISH
Bud germ ring ISH
WT + MO1-fgfr1a chemical treatment: all-trans-retinoic acid Bud forerunner cell group ISH
Bud germ ring ISH
WT + MO1-fgfr1a standard conditions Bud forerunner cell group ISH
Bud germ ring ISH
WT + MO1-tbxta standard conditions Bud forerunner cell group ISH
Bud germ ring ISH
WT + MO1-wnt8a chemical treatment: 4-(diethylamino)benzaldehyde Bud forerunner cell group ISH
Bud germ ring ISH
WT + MO1-wnt8a standard conditions Bud forerunner cell group ISH
Bud germ ring ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 374(1), Qian, M., Yao, S., Jing, L., He, J., Xiao, C., Zhang, T., Meng, W., Zhu, H., Xu, H., and Mo, X., ENC1-like Integrates the Retinoic Acid/FGF Signaling Pathways to Modulate Ciliogenesis of Kupffer's Vesicle during Zebrafish Embryonic Development, 85-95, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.