ZFIN ID: ZDB-FIG-121101-40
Lin et al., 2012 - Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio). PLoS One   7(9):e45650 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 5 Effects of vitamin D receptor (VDR)a and VDRb morpholino oligonucleotides (MOs) in 3-d post-fertilization (dpf) zebrafish embryos.

Ca2+ influx (A), Ca2+ content (B), mRNA expression (C), density of ecac-expressing cells (D), and ecac signals (E). mRNA expressions were analyzed by a qPCR using β-actin as the internal control. Different letters indicate a significant difference (p<0.05) using a one-way ANOVA followed by Tukey’s multiple-comparison test. Values are the mean ± SD (n = 6 or 7). Scale bar:100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atp2b2 AB control Protruding-mouth whole organism RTPCR
AB + MO1-vdra standard conditions Protruding-mouth whole organism RTPCR
AB + MO1-vdrb standard conditions Protruding-mouth whole organism RTPCR
slc8a1b AB control Protruding-mouth whole organism RTPCR
AB + MO1-vdra standard conditions Protruding-mouth whole organism RTPCR
AB + MO1-vdrb standard conditions Protruding-mouth whole organism RTPCR
trpv6 AB control Protruding-mouth integument ISH
Protruding-mouth whole organism RTPCR
AB + MO1-vdra standard conditions Protruding-mouth integument ISH
Protruding-mouth whole organism RTPCR
AB + MO1-vdrb standard conditions Protruding-mouth integument ISH
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-vdra standard conditions Protruding-mouth calcium ion import decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One