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ZFIN ID: ZDB-FIG-121101-11
Weiss et al., 2012 - Abnormal vasculature interferes with optic fissure closure in lmo2 mutant zebrafish embryos. Developmental Biology   369(2):191-198 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Pec-fin

Fig. 2 Edema and ocular vessel phenotypes in lmo2 mutant embryos. (A and B) Transverse sections of heads from normal (A) and lmo2 mutant (B) embryos at 2.5 dpf. In the mutant note enlarged ventricle and white spaces within head and eye (arrow) tissues suggesting severe edema. Asterisks mark brain ventricle. (C and D) Three-dimensional shadow rendering confocal images of 55ā€“56 hpf Tg(kdrl:EGFP) normal (C) and lmo2 mutant (D) eyes in frontal view. (E and F) Three-dimensional maximum projection confocal images of 55ā€“56 hpf Tg(kdrl:EGFP) normal (E) and lmo2 mutant (F) eyes in lateral view. Arrows point at different regions of the superficial ring vessel. (G and H) FITC-dextran microangiography of Tg(kdrl:HsHRAS-mCherry) normal (G) and lmo2 mutant (H) eyes in live embryos at 1.5 dpf, lateral view. Note accumulation of dye around the mutant eye (arrowheads). drv, dorsal radial vessel; ha, hyaloid artery; hc, hyaloid capillaries; hv, hyaloid vein; nrv, nasal radial vessel; vrv, ventral radial vessel. In (Eā€“H) anterior is to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP lmo2vu270/vu270; s843Tg standard conditions Long-pec ocular blood vessel blood vessel endothelium IHC
s843Tg standard conditions Long-pec ocular blood vessel blood vessel endothelium IHC
mCherry lmo2vu270/vu270; s896Tg standard conditions Prim-25 ocular blood vessel blood vessel endothelium IHC
s896Tg standard conditions Prim-25 ocular blood vessel blood vessel endothelium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lmo2vu270/vu270(AB) standard conditions Pec-fin eye edematous, abnormal
Pec-fin head edematous, abnormal
Pec-fin ventricular system edematous, abnormal
lmo2vu270/vu270; s843Tg standard conditions Long-pec hyaloid artery constricted, abnormal
Long-pec hyaloid vein dilated, abnormal
Long-pec retinal blood vessel morphogenesis decreased process quality, abnormal
lmo2vu270/vu270; s896Tg standard conditions Prim-25 ocular blood vessel dilated, abnormal
lmo2vu270/vu270; s896Tg standard conditions Prim-25 ocular blood vessel increased permeability, abnormal
lmo2vu270/vu270; s896Tg standard conditions Prim-25 retinal blood vessel morphogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Weiss, O., Kaufman, R., Michaeli, N., and Inbal, A., Abnormal vasculature interferes with optic fissure closure in lmo2 mutant zebrafish embryos, 191-198, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.