FIGURE

Fig. 11

ID

ZDB-FIG-121004-26

Publication

An et al., 2012 - The zebrafish sf3b1(b460) mutant reveals differential requirements for the sf3b1 pre-mRNA processing gene during neural crest development

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Fig. 11

Analysis of cell death in sf3b1^b460 mutants. (A-C) Double labeling with TUNEL (NBT/BCIP) and crestin whole-mount in situ hybridization (fast red). Transverse section is from the trunk region of a sf3b1^b460 mutant, dorsal is up. (A) DIC image/TUNEL; (B) fluorescence image/crestin of the same section; (C) (A,B) merged. (D) Dorsal view of uninjected sf3b1^b460 mutant at 16 hpf with TUNEL staining. (E) Dorsal view of zbcl2 mRNA injected sf3b1^b460 mutant at 16 hpf with TUNEL staining. Reduced TUNEL staining is evident on the left side. (F) Lateral view of live uninjected sf3b1^b460 mutant at 28 hpf. (G) Lateral view of live zbcl2 injected sf3b1^b460 mutant at 28 hpf, showing less CNS cell death as compared with uninjected sf3b1^b460 mutants but no melanophore rescue.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sf3b1^b460/b460
Observed In:	brain neural crest post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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