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ZFIN ID: ZDB-FIG-120601-79
van der Velden et al., 2012 - The Polycomb group protein Ring1b is essential for pectoral fin development. Development (Cambridge, England)   139(12):2210-2220 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 8 Restoration of mesenchymal gene expression and fin bud outgrowth in apc/ring1b mutants. (A-X) Dorsal views of embryos of the indicated genotypes stained for tbx5, fgf24 and fgf10 at 32 hpf (A-L) and 72 hpf (M-X). tbx5 (B,N), fgf24 (F,R) and fgf10 (J,V) are overexpressed in the apc pectoral fin mesenchyme at both 32 and 72 hpf. Expression of fgf24 (H) and fgf10 (L) is increased in apc/ring1b mutants compared with ring1b mutants (G,K) at 32 hpf. Expression of tbx5 (P), fgf24 (T) and fgf10 (X) is maintained in apc/ring1b mutants at 72 hpf. Arrowheads indicate staining at the fin bud region. (Y-BB) Lateral views of 72 hpf embryos of the indicated genotypes at the level of the pectoral fin. A rudimentary fin bud (outlined) is formed in apc/ring1b mutants at 72 hpf (BB).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fgf10a WT standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
apchu745/hu745 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
apchu745/hu745; rnf2ibl30/ibl30 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
apchu745/hu745; rnf2ibl31/ibl31 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
rnf2ibl30/ibl30 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth Not Detected pectoral fin ISH
rnf2ibl31/ibl31 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth Not Detected pectoral fin ISH
fgf24 WT standard conditions Prim-15 mesenchyme pectoral fin ISH
Protruding-mouth mesenchyme pectoral fin ISH
apchu745/hu745 standard conditions Prim-15 mesenchyme pectoral fin ISH
Protruding-mouth mesenchyme pectoral fin ISH
apchu745/hu745; rnf2ibl30/ibl30 standard conditions Prim-15 mesenchyme pectoral fin ISH
Protruding-mouth mesenchyme pectoral fin ISH
apchu745/hu745; rnf2ibl31/ibl31 standard conditions Prim-15 mesenchyme pectoral fin ISH
Protruding-mouth mesenchyme pectoral fin ISH
rnf2ibl30/ibl30 standard conditions Prim-15 mesenchyme pectoral fin ISH
Protruding-mouth Not Detected pectoral fin ISH
rnf2ibl31/ibl31 standard conditions Prim-15 mesenchyme pectoral fin ISH
Protruding-mouth Not Detected pectoral fin ISH
tbx5a WT standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
apchu745/hu745 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
apchu745/hu745; rnf2ibl30/ibl30 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
apchu745/hu745; rnf2ibl31/ibl31 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth pectoral fin ISH
rnf2ibl30/ibl30 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth Not Detected pectoral fin ISH
rnf2ibl31/ibl31 standard conditions Prim-15 pectoral fin ISH
Protruding-mouth Not Detected pectoral fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apchu745/hu745 standard conditions Protruding-mouth pectoral fin decreased length, abnormal
Protruding-mouth pectoral fin ruffled, abnormal
apchu745/hu745; rnf2ibl30/ibl30 standard conditions Protruding-mouth pectoral fin decreased length, abnormal
Protruding-mouth pectoral fin ruffled, abnormal
apchu745/hu745; rnf2ibl31/ibl31 standard conditions Protruding-mouth pectoral fin decreased length, abnormal
Protruding-mouth pectoral fin ruffled, abnormal
rnf2ibl30/ibl30 standard conditions Protruding-mouth pectoral fin absent, abnormal
rnf2ibl31/ibl31 standard conditions Protruding-mouth pectoral fin absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development