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ZIRC
ZFIN ID: ZDB-FIG-120315-45
Simmons et al., 2012 - Mutation of pescadillo Disrupts Oligodendrocyte Formation in Zebrafish. PLoS One   7(2):e32317 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 1

Characterization and genetic mapping of the vu166 mutant allele.

Lateral views of 4 dpf wild-type (A,C) and vu166 -/- larvae (B,D). EGFP expression revealed oligodendrocytes in dorsal spinal cord of wild-type Tg(olig2:EGFP) larva (C, bracket). Fewer dorsal EGFP+ cells were evident in a vu166 -/- larva (D). Bulked segregant analysis revealed that the vu166 allele links to chromosome 5 (E). The recombination frequencies (i) at various microsatellite markers (ii) at known locations (iii) along chromosome 5 show that the mutation lies near the pescadillo (pes) gene locus. The vu166 allele failed to complement the previously characterized peshi2 mutant allele, producing phenotypically wild-type (F) and mutant (G) larvae in a 3:1 ratio. The Pes gene product (H) contains a highly conserved Pescadillo Homology domain, a BRCA1 C-terminal domain, two Acidic Domains (AD), and a Nuclear Localization Sequence (NLS). Sequencing of pes cDNA obtained from vu166 mutants revealed a single base pair mutation that creates a premature stop codon predicted to prematurely truncate the protein.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pesvu166/vu166; vu12Tg standard conditions Day 4 oligodendrocyte IFL
Day 4 spinal cord dorsal region IFL
vu12Tg standard conditions Day 4 oligodendrocyte IFL
Day 4 spinal cord dorsal region IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pesvu166/+; peshi2Tg/+; vu12Tg standard conditions Day 4 eye decreased size, abnormal
Day 4 forebrain morphology, abnormal
Day 4 mandibular arch skeleton decreased size, abnormal
Day 4 otolith morphology, abnormal
Day 4 pectoral fin morphology, abnormal
Day 4 spinal cord dorsal region has fewer parts of type oligodendrocyte, abnormal
Day 4 whole organism decreased length, abnormal
pesvu166/vu166; vu12Tg standard conditions Day 4 eye decreased size, abnormal
Day 4 forebrain morphology, abnormal
Day 4 mandibular arch skeleton decreased size, abnormal
Day 4 otolith morphology, abnormal
Day 4 pectoral fin morphology, abnormal
Day 4 spinal cord dorsal region has fewer parts of type oligodendrocyte, abnormal
Day 4 whole organism decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One