ZFIN ID: ZDB-FIG-120227-6
Odenthal et al., 1996 - Mutations affecting the formation of the notochord in the zebrafish, Danio rerio. Development (Cambridge, England)   123:103-115 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Bud to 14-19 somites

Fig. 6

Dorsal view of myoD RNA (blue staining) and Ntl antibody staining (brown nuclear staining) at (A-G) tailbud stage and (H-M) 15- somite stage of (A,H) wild-type, (B,I) flhtk241, (C,J) momth211, (D,K) ntltc41, (E) ntlb160, (F) ntltb244e, (L) ntlts260 and (G,M) doctt258 embryos. (A) In wild type at the tailbud stage myoD expression is restricted to a line of cells left and right of the notochord. (B,C) The gap between the two stripes is filled with myoD expressing cells in (B) flhtk241 and (C) momth211 embryos. ntl expression is detectable only in the tailbud and sometimes in a few cells anteriorly, which do not express myoD (C). (D) Strongly reduced myoD expression a greater distance from the midline is detectable in embryos with the strong allele, ntltc41(arrows). myoD expression at the 15-somite stage in (H) wild type is expressed in the adaxial cells and in the posterior part of the somites. In (I) flhtk241 embryos and (J) momth211 embryos myoD expression in the adaxial cells is not maintained. (K) In embryos with the strong allele, ntltc41, somites are separated by a big gap of nonexpressing cells, whereas the 15th somite is fused in the midline (arrow). (L) In embryos with the weak allele, ntlts260, adaxial cells next to ntl-expressing cells express myoD at a higher level than the somites on the opposite side. (M) In doctt258 embryos adaxial expression in anterior regions is reduced and closer together.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 WT standard conditions Bud adaxial cell ISH
14-19 somites adaxial cell ISH
14-19 somites somite ISH
dharmath211/th211 standard conditions Bud adaxial cell ISH
Bud axial mesoderm ISH
14-19 somites Not Detected adaxial cell ISH
14-19 somites somite ISH
doctt258/tt258 standard conditions Bud adaxial cell ISH
14-19 somites adaxial cell ISH
14-19 somites somite ISH
nototk241/tk241 standard conditions Bud adaxial cell ISH
Bud axial mesoderm ISH
14-19 somites Not Detected adaxial cell ISH
14-19 somites somite ISH
tbxtab160/b160 (AB) standard conditions Bud adaxial cell ISH
tbxtatb244e/tb244e standard conditions Bud adaxial cell ISH
tbxtatc41/tc41 standard conditions Bud adaxial cell ISH
14-19 somites Not Detected adaxial cell ISH
14-19 somites somite ISH
tbxtats260/ts260 standard conditions 14-19 somites adaxial cell ISH
14-19 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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