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ZFIN ID: ZDB-FIG-120209-9
Hall et al., 2012 - Infection-Responsive Expansion of the Hematopoietic Stem and Progenitor Cell Compartment in Zebrafish Is Dependent upon Inducible Nitric Oxide. Cell Stem Cell   10(2):198-209 Full text @ Cell Stem Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 4

Fig. 3

Demand-Driven Granulopoiesis Is Dependent upon Gcsf/Gcsfr Signaling (A) Expression of gcsf within the midbrain and hindbrain of infected larvae (marked by arrows), dorsal views, compared with PBS controls at 1 and 2 dpi. (B) Expression of gcsfr within the AGM of infected larvae, compared with PBS controls at 1 and 2 dpi. (C–E) Dual WMISH of gcsf and lyz, and lcp1 and apoeb, respectively, within the midbrain and hindbrain at 2 dpi following infection, with dorsal views shown. (F) Control MO- and Gcsfr MO-injected Tg(lyz:EGFP)/Tg(gata1:DsRED) larvae following injection of heat-shocked Salmonella (H.S. Sal.), compared with PBS controls at 2 dpi. Numbers represent frequency of embryos or larvae with displayed phenotype. Scale bar, 100 μm in (F). See also Figure S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
apoeb WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 microglial cell ISH
csf3r WT standard conditions Day 4 Not Detected ventral wall of dorsal aorta ISH
WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 leukocyte ISH
Day 4 macrophage ISH
Day 4 Not Detected microglial cell ISH
Day 4 neutrophil ISH
Day 4 ventral wall of dorsal aorta cell ISH
EGFP nz117Tg; sd2Tg standard conditions Day 4 neutrophil IFL
nz117Tg; sd2Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 neutrophil IFL
nz117Tg; sd2Tg + MO4-csf3r standard conditions Day 4 neutrophil IFL
nz117Tg; sd2Tg + MO4-csf3r bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 neutrophil IFL
lcp1 WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 leukocyte ISH
lyz WT bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz117Tg; sd2Tg bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Day 4 granulocyte differentiation increased occurrence, abnormal
Day 4 neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Stem Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Stem Cell