FIGURE

Fig. 6

ID

ZDB-FIG-111220-8

Publication

Doll et al., 2011 - Subnuclear development of the zebrafish habenular nuclei requires ER translocon function

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Fig. 6

Increased cell division of LsDh precursors in sec61al1^c163 mutants. Embryos were briefly pulsed with BrdU at 32 hpf and chased until 4 dpf, when they were labeled for Kctd12.1 (green) and BrdU (red). As neurons are post-mitotic, brightly labeled BrdU+ cells are those that underwent a final mitosis during the BrdU pulse (white arrows); see text for further explanation of the labeling strategy. (A–A′′′) Compared to WT controls, (B–B′′′) significantly more neurons are born in the RHb of sec61al1^c163 mutants at 32 hpf. This trend continues through 48 hpf (A′′′,B′′′), as again there are significantly more neurons in the RHb of sec61al1^c163 mutants (images not shown) (Andres et al., 1999). Images are single z-plane images from confocal z-stacks. Dorsal (A,B), mid (A′,B′), and ventral (A′′,B′′) sections are shown. Scale bar represents 30 μm. Significance determined by Student′s T-test (*p < 0.05; **p < 0.01); n = 41 siblings, n = 43 mutants.

Expression Data

Antibody:	Ab1-elavl
Fish:	AB sec61a1a^c163/c163 (AB)
Anatomical Terms:	dorsal habenular nucleus lateral region dorsal habenular nucleus neuron
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sec61a1a^c163/c163 (AB)
Observed In:	cell division dorsal habenular nucleus lateral region neuron
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 360(1), Doll, C.A., Burkart, J.T., Hope, K.D., Halpern, M.E., and Gamse, J.T., Subnuclear development of the zebrafish habenular nuclei requires ER translocon function, 44-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.