ZFIN ID: ZDB-FIG-111102-15
Expression of vegfc is specifically regulated by Wnt/β-catenin signaling. (A-D) In situ hybridization of the mid-trunk of 24 hpf of wild-type sibling (A,C), etsrpy11 mutant (B) and flky17 mutant (D) embryos, probing for vegfc. Arrows show vegfc expression in the dorsal aorta, which is not affected in the two mutants. (E,F) RT-PCR analysis showing expression of vegfc by cultured mouse endothelial cells upon treatment with BIO (E) or Wnt3a (F). (G,H) In situ hybridization of 26 hpf rspo1 MO-injected embryos treated from 16-26 hpf with either 0.5% DMSO carrier (G) or 0.5 μM BIO in DMSO (H), probed for vegfc (arrows). (I,J) In situ hybridization of 36 hpf wild-type sibling (I) or homozygous (apcmcr/apcmcr) mutant (J) embryos probed for vegfc (arrows). Scale bars: 50 μm.
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