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ZFIN ID: ZDB-FIG-111102-15
Gore et al., 2011 - Rspo1/Wnt signaling promotes angiogenesis via Vegfc/Vegfr3. Development (Cambridge, England)   138(22):4875-4886 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 6

Expression of vegfc is specifically regulated by Wnt/β-catenin signaling. (A-D) In situ hybridization of the mid-trunk of 24 hpf of wild-type sibling (A,C), etsrpy11 mutant (B) and flky17 mutant (D) embryos, probing for vegfc. Arrows show vegfc expression in the dorsal aorta, which is not affected in the two mutants. (E,F) RT-PCR analysis showing expression of vegfc by cultured mouse endothelial cells upon treatment with BIO (E) or Wnt3a (F). (G,H) In situ hybridization of 26 hpf rspo1 MO-injected embryos treated from 16-26 hpf with either 0.5% DMSO carrier (G) or 0.5 μM BIO in DMSO (H), probed for vegfc (arrows). (I,J) In situ hybridization of 36 hpf wild-type sibling (I) or homozygous (apcmcr/apcmcr) mutant (J) embryos probed for vegfc (arrows). Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
vegfc WT standard conditions Prim-5 dorsal aorta ISH
Prim-25 dorsal aorta ISH
WT + MO3-rspo1 chemical treatment: pharmaceutical Prim-5 dorsal aorta ISH
WT + MO3-rspo1 standard conditions Prim-5 Not Detected dorsal aorta ISH
apchu745/hu745 standard conditions Prim-25 dorsal aorta ISH
etv2y11/y11 standard conditions Prim-5 dorsal aorta ISH
kdrly17/y17 standard conditions Prim-5 dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
etv2y11/y11 standard conditions Prim-5 angiogenesis disrupted, abnormal
Prim-5 axial blood vessel morphology, normal
Prim-5 intersegmental vessel morphology, abnormal
kdrly17/y17 standard conditions Prim-5 angiogenesis disrupted, abnormal
Prim-5 axial blood vessel morphology, normal
Prim-5 intersegmental vessel morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development