ZFIN ID: ZDB-FIG-111028-29
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5 The altered balance of alpha and beta cells in Mnx1-deficient embryos is maintained after ventral bud development. Confocal images of whole-mount 72 hpf Tg(neurod:EGFP) zebrafish embryos that were (A,E) uninjected, or injected with (B,F) control MO, (C,G) Mnx1 MO1 or (D,H) Mnx1 MO2, immunolabeled for glucagon (red), insulin (blue) and GFP (green) as indicated. Data are representative of three independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg control Protruding-mouth endocrine pancreas IHC
nl1Tg + MO1-mnx1 standard conditions Protruding-mouth endocrine pancreas IHC
nl1Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas IHC
ins Ab1-ins nl1Tg control Protruding-mouth endocrine pancreas pancreatic B cell IHC
Ab1-ins nl1Tg + MO1-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic B cell IHC
Ab1-ins nl1Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic B cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gcg IHC nl1Tg control Protruding-mouth endocrine pancreas pancreatic A cell
IHC nl1Tg + MO1-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic A cell
IHC nl1Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas pancreatic A cell
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg + MO1-mnx1 standard conditions Protruding-mouth endocrine pancreas has extra parts of type pancreatic A cell, abnormal
Protruding-mouth endocrine pancreas has fewer parts of type pancreatic B cell, abnormal
nl1Tg + MO2-mnx1 standard conditions Protruding-mouth endocrine pancreas has extra parts of type pancreatic A cell, abnormal
Protruding-mouth endocrine pancreas has fewer parts of type pancreatic B cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development