ZFIN ID: ZDB-FIG-111028-27
Dalgin et al., 2011 - Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England)   138(21):4597-4608 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 3 Endocrine progenitors are unaffected by Mnx1 knockdown. (A-D) Confocal images of Tg(neurod:EGFP) zebrafish embryos at 18 hpf. Whole-mount immunolabeling for GFP (green) and for myosin to label somites (blue); nuclear staining with TO-PRO-3 (red). Somites are numbered from anterior to posterior (S1-S6). (E-H) In situ hybridization for neurod at 18 hpf. (A,E) Uninjected controls; (B,F) control morphants; (C,G) Mnx1 MO1-injected embryos; (D,H) Mnx1 MO2-injected embryos. (I) Mean (± s.d.) neurod-expressing cells from two independent experiments and from a minimum of 17 embryos per group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg control 14-19 somites endoderm endocrine cell IHC
nl1Tg + MO1-mnx1 standard conditions 14-19 somites endoderm endocrine cell IHC
nl1Tg + MO2-mnx1 standard conditions 14-19 somites endoderm endocrine cell IHC
myod1 nl1Tg control 14-19 somites somite IHC
nl1Tg + MO1-mnx1 standard conditions 14-19 somites somite IHC
nl1Tg + MO2-mnx1 standard conditions 14-19 somites somite IHC
neurod1 nl1Tg control 14-19 somites endoderm endocrine cell ISH
nl1Tg + MO1-mnx1 standard conditions 14-19 somites endoderm endocrine cell ISH
nl1Tg + MO2-mnx1 standard conditions 14-19 somites endoderm endocrine cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg + MO1-mnx1 standard conditions 14-19 somites endoderm has normal numbers of parts of type endocrine cell, normal
nl1Tg + MO2-mnx1 standard conditions 14-19 somites endoderm has normal numbers of parts of type endocrine cell, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development