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ZFIN ID: ZDB-FIG-111014-32
Lu et al., 2011 - Identification and mechanism of regulation of the zebrafish dorsal determinant. Proceedings of the National Academy of Sciences of the United States of America   108(38):15876-80 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-cell to Prim-5

Fig. 3 Negative regulation of the canonical Wnt pathway by Sfrp1a and Frzb. Immunofluorescence localization of β-catenin is shown at 3 hpf (high stage) (A), at the dorsal margin (DM), and (B) at the animal pole (AP) of a wild-type embryo or (C) at the animal pole of an embryo overexpressing Wnt8a (10 pg Wnt8a mRNA). White and yellow arrowheads in A point to the accumulation of β-catenin in blastomeres and yolk syncytial layer nuclei, respectively. (D) Analysis by RT-PCR of the expression of zebrafish Wnt inhibitors during early development. Sfrp (TLC), expressed only during gastrulation (35), is not presented here. Expression of chordin at the sphere stage in lateral view (Upper) and animal pole view (Lower) in (E and I) wild-type embryos, (F and J) Sfrp1a morphants, (G and K) Frzb morphants, and (H and L) Sfrp1a–Frzb double morphants. (MO) Immunofluorescence localization of β-catenin in nuclei of the animal pole of embryos at the high stage in (J) Sfrp1a, (K) Frzb, and (L) Sfrp1a–Frzb morphants. Embryos are in dorsal view (A), lateral view (EH), and in animal pole view (B, C, and IO). (Scale bar, 100 μM.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
chrd WT standard conditions Sphere blastodisc ISH
Sphere whole organism ISH
WT + MO1-frzb standard conditions Sphere whole organism ISH
WT + MO1-frzb + MO1-sfrp1a standard conditions Sphere whole organism ISH
WT + MO1-sfrp1a standard conditions Sphere whole organism ISH
dkk1a WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
dkk1b WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
dkk2 WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
30%-epiboly Not Detected whole organism RTPCR
Sphere Not Detected whole organism RTPCR
Shield Not Detected whole organism RTPCR
Prim-5 whole organism RTPCR
dkk3b WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
frzb WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
sfrp1a WT standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
sfrp1b WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
sfrp2 WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield Not Detected whole organism RTPCR
Prim-5 whole organism RTPCR
sfrp2l WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
30%-epiboly whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
sfrp5 WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
30%-epiboly Not Detected whole organism RTPCR
Sphere Not Detected whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
wif1 WT standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
Sphere whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism RTPCR
Prim-5 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ctnnb IHC WT standard conditions High blastomere nucleus
IHC High embryonic structure dorsal margin
IHC High yolk syncytial layer nucleus
IHC WT + MO1-frzb standard conditions High blastomere nucleus
IHC High embryonic structure dorsal margin
IHC High yolk syncytial layer nucleus
IHC WT + MO1-frzb + MO1-sfrp1a standard conditions High blastomere nucleus
IHC High embryonic structure dorsal margin
IHC High yolk syncytial layer nucleus
IHC WT + MO1-sfrp1a standard conditions High blastomere nucleus
IHC High embryonic structure dorsal margin
IHC High yolk syncytial layer nucleus
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA