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ZFIN ID: ZDB-FIG-110915-7
Solomon et al., 2003 - Zebrafish foxi1 mediates otic placode formation and jaw development. Development (Cambridge, England)   130(5):929-940 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Day 5

Fig. 8 Analysis of jaw and neural crest in hsy. (A,C,E,G) Wild-type and (B,D,F,H) hsy mutant embryos. (A,B) Alcian Green staining of cartilaginous jaw elements at 5 days. Ventral views, anterior towards the left. Cartilages: bh, basihyal; ch, ceratohyal; m, mandibular; pq, palatoquadrate; 3-7, gill cartilages derived from branchial arches P3- P7. m and pq are P1 derivatives; ch and bh are derived from P2. ch+3 indicates unilateral fusion of ceratohyal with gill arch 3 in mutant. (C-H) Expression analysis at the 18-somite stage: dorsal views, anterior towards the top. (C,D) Double labeling with crestin (dark purple) and krox 20 (red). (E,F) dlx2a expression. (G,H) foxi1 expression. (I) TaqI restriction polymorphism genotyping data for foxi1 in situ hybridization at the 18-somite stage, sorted as either ‘upregulated’ or ‘normal expression’. m, mandibular neural crest (nc) stream; h, hyoid nc; v, vagal nc; 3 and 5, krox20 expression in rhombomeres 3 and 5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crestin WT standard conditions 14-19 somites hyoid neural crest ISH
14-19 somites mandibular neural crest ISH
14-19 somites vagal neural crest ISH
foxi1em1/em1 standard conditions 14-19 somites hyoid neural crest ISH
14-19 somites mandibular neural crest ISH
14-19 somites vagal neural crest ISH
dlx2a WT standard conditions 14-19 somites hyoid neural crest ISH
14-19 somites mandibular neural crest ISH
14-19 somites vagal neural crest ISH
foxi1em1/em1 standard conditions 14-19 somites hyoid neural crest ISH
14-19 somites mandibular neural crest ISH
14-19 somites vagal neural crest ISH
foxi1 WT standard conditions 14-19 somites cranial neural crest cell ISH
foxi1em1/em1 standard conditions 14-19 somites cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxi1em1/em1 standard conditions 14-19 somites vagal neural crest decreased distance hyoid neural crest, abnormal
Day 5 ceratohyal cartilage decreased length, abnormal
Day 5 ceratohyal cartilage increased thickness, abnormal
Day 5 ceratohyal cartilage orientation ceratohyal cartilage, abnormal
Day 5 embryonic viscerocranium morphogenesis disrupted, abnormal
Day 5 pharyngeal arch morphology, abnormal
Day 5 pharyngeal arch 3-7 orientation whole organism anterior-posterior axis, abnormal
Day 5 pharyngeal arch anterior-posterior axis decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development