ZFIN ID: ZDB-FIG-110622-2
Colocalization of Collagen type I and Type II in actinotrichia during development and regeneration. Immunofluorescence against Collagen I (red) and Collagen II (green). Sequential confocal sections were obtained in the Z-axis of the whole thick of the samples. Projections of the Z-stack are shown for the red (A), the green (B) and the merged channels (C) of the fin fold of a doubly immunostained 72 hpf embryo. A confocal plane of the merged red and green channels, at a higher magnification, shows the actinotrichia fibers in detail (D). Z-stack projections of all confocal sections .of a 4 dpa longitudinal fin section doubly immunostained and with nuclei counterstained in blue (E–G). E: Red and blue channels. F: Green and blue channels. G: All-merged channels. H: A detail of all merged channels shows colocalization of the green and red labels. Bars represent 100 μm in A–C and E–G; and 25 μm in D and H. Arrowhead is actinotrichia. e is epidermis. b is basal epidermal layer. c is connective tissue. n is notochord.
Gene Expression DetailsNo data available
Antibody Labeling Details
Phenotype Details No data available
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
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Reprinted from Developmental Biology, 354(1), Durán, I., Marí-Beffa, M., Santamaría, J.A., Becerra, J., and Santos-Ruiz, L., Actinotrichia collagens and their role in fin formation, 160-172, Copyright
(2011) with permission from Elsevier.
Full text @ Dev. Biol.