header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-110622-2
Durán et al., 2011 - Actinotrichia collagens and their role in fin formation. Developmental Biology   354(1):160-172 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Term:
Stage Range: Protruding-mouth to Day 4

Fig. 2

Colocalization of Collagen type I and Type II in actinotrichia during development and regeneration. Immunofluorescence against Collagen I (red) and Collagen II (green). Sequential confocal sections were obtained in the Z-axis of the whole thick of the samples. Projections of the Z-stack are shown for the red (A), the green (B) and the merged channels (C) of the fin fold of a doubly immunostained 72 hpf embryo. A confocal plane of the merged red and green channels, at a higher magnification, shows the actinotrichia fibers in detail (D). Z-stack projections of all confocal sections .of a 4 dpa longitudinal fin section doubly immunostained and with nuclei counterstained in blue (E–G). E: Red and blue channels. F: Green and blue channels. G: All-merged channels. H: A detail of all merged channels shows colocalization of the green and red labels. Bars represent 100 μm in A–C and E–G; and 25 μm in D and H. Arrowhead is actinotrichia. e is epidermis. b is basal epidermal layer. c is connective tissue. n is notochord.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col1a1 IHC AB standard conditions Protruding-mouth actinotrichium
IHC AB physical alteration: anatomical structure Day 4 actinotrichium
Ab1-col2a IHC AB standard conditions Protruding-mouth actinotrichium
IHC AB physical alteration: anatomical structure Day 4 actinotrichium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 354(1), Durán, I., Marí-Beffa, M., Santamaría, J.A., Becerra, J., and Santos-Ruiz, L., Actinotrichia collagens and their role in fin formation, 160-172, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.