header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-110622-100
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.
Nevin et al., 2011 - Topoisomerase II{beta} is required for lamina-specific targeting of retinal ganglion cell axons and dendrites. Development (Cambridge, England)   138(12):2457-2465 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 3

noto mutant tectal neurons have unrefined neuropil projections at 5 dpf. Sections of wild-type (A,C,E) and mutant (B,D,F) zebrafish tectum showing tectal cell populations and their neuropil neurites. Disorganized neuropil in the mutant is marked with asterisks. (A,B) Wild-type Parvalbumin (Parv)+ tectal neurons project neurites to four laminae in the neuropil; this laminar organization is incomplete in the mutant. (C,D) Wild-type Protein kinase C (PKC)+ tectal neurons project neurites to three laminae in the neuropil; this laminar organization is lost in the mutant. (E,F) Merged images showing relative location of laminae. Scale bar: 50 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-prkcb1 IHC WT standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
IHC top2bs380/s380(TL) standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
Ab1-pvalb IHC WT standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
IHC top2bs380/s380(TL) standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
Phenotype Details
Fish Conditions Stage Phenotype
top2bs380/s380(TL) standard conditions Day 5 optic tectum neuron projection disorganized, abnormal
Day 5 optic tectum neuron projection mislocalised, abnormal
Day 5 retinal ganglion cell axon guidance disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development