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ZIRC
ZFIN ID: ZDB-FIG-110622-100
Nevin et al., 2011 - Topoisomerase II{beta} is required for lamina-specific targeting of retinal ganglion cell axons and dendrites. Development (Cambridge, England)   138(12):2457-2465 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 3

noto mutant tectal neurons have unrefined neuropil projections at 5 dpf. Sections of wild-type (A,C,E) and mutant (B,D,F) zebrafish tectum showing tectal cell populations and their neuropil neurites. Disorganized neuropil in the mutant is marked with asterisks. (A,B) Wild-type Parvalbumin (Parv)+ tectal neurons project neurites to four laminae in the neuropil; this laminar organization is incomplete in the mutant. (C,D) Wild-type Protein kinase C (PKC)+ tectal neurons project neurites to three laminae in the neuropil; this laminar organization is lost in the mutant. (E,F) Merged images showing relative location of laminae. Scale bar: 50 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-prkcb1 IHC WT standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
IHC top2bs380/s380(TL) standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
Ab1-pvalb IHC WT standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
IHC top2bs380/s380(TL) standard conditions Day 5 optic tectum axon
IHC Day 5 optic tectum CNS neuron (sensu Vertebrata)
Phenotype Details
Fish Conditions Stage Phenotype
top2bs380/s380(TL) standard conditions Day 5 optic tectum neuron projection disorganized, abnormal
Day 5 optic tectum neuron projection mislocalised, abnormal
Day 5 retinal ganglion cell axon guidance disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development