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ZIRC
ZFIN ID: ZDB-FIG-110608-6
Chung et al., 2011 - Zebrafish her8a is activated by Su(H)-dependent Notch signaling and is essential for the inhibition of neurogenesis. PLoS One   6(4):e19394 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-25

Fig. 7 Glial precursors and mature glial cells were reduced in Her8a knockdown embryos.

Her8a morpholino injection downregulates the expression of the glial precursor marker slc1a3a (A, B) and radial glial markers (gfap for radial glial cell body (D, E) and zrf-1 for glial fibers (G, H)), and this effect was rescued by co-injection with her8a mRNA (C, F, I). (A, D, G) Control morpholino, (B, E, H) 8 ng of MO2, (C, F, I) Co-injection of 8 ng of MO2 with 0.5 ng her8a mRNA. Embryos were harvested at the 18-somite stage (A–C) and 36 hpf (D–I). (A–F) in situ hybridization; (G–I) immunohistochemistry with zrf-1 antibody, fluorescent signals were inverted to negative film for a better presentation. (J) qPCR analysis showing the levels of slc1a3a and zash1 and zrf-1 are significantly decreased in embryos injected with her8a morpholino. * p<0.05.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gfap TU control Prim-25 radial glial cell ISH
TU + MO2-her8a standard conditions Prim-25 radial glial cell ISH
slc1a3a TU control 14-19 somites glioblast ISH
TU + MO2-her8a standard conditions 14-19 somites glioblast ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zrf-1 IHC TU standard conditions Prim-25 radial glial cell
IHC TU + MO2-her8a standard conditions Prim-25 radial glial cell
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO2-her8a standard conditions 14-19 somites - Prim-25 gliogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One