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ZFIN ID: ZDB-FIG-110527-12
Wang et al., 2011 - Zebrafish Thsd7a is a neural protein required for angiogenic patterning during development. Developmental dynamics : an official publication of the American Association of Anatomists   240(6):1412-1421 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 5 thsd7a expression is associated with ISV development. A–F: Expression of thsd7a transcript (green) was detected by fluorescent ISH, followed by immunostaining to reveal the ISV pattern (red). Anterior is to the left. Expression of thsd7a in the ventral edge of neural tube is indicated by white arrows. Lateral deviation is indicated by white arrowheads. Scale bars represent 50 μm. Representative images showing the control embryo (A–C), and morphant at 30 hours postfertilization (hpf; D–F). G–L: Transplanted wild-type cells rescue the ISV phenotype. Embryos were examined at 28 hpf (G–I) and 50 hpf (J–L). ISVs were shown in green (G,J). Clusters of dextran-labeled cells were shown in red (H,K). G: ISVs that reached the dorsal region of the embryo were indicated by asterisk. H: Clusters of dextran-labeled cells were found in the recipients. I: Merged image of panel G and H. J,K: By 50 hpf, arrowheads indicate the normal ISVs that fused adjacently at the dorsal region of the embryo. Dextran-labeled cells were found associated with the normal ISVs. L: Images were merged. M,N: Models for the role of Thsd7a in EC migration during ISV angiogenesis. M: Thsd7a may be a membrane-anchored protein on the surface of neural cells, which distribute predominantly along the ventral edge of neural tube and located on the growth path of angiogenic ISVs. Interaction between Thsd7a and EC receptors directs EC migration during ISV angiogenesis. N: Fragments of Thsd7a may be released from neural cells, and form a concentration gradient that guide EC migration during ISV angiogenesis. NT, neural tube; N, notochord; DA, dorsal aorta; ISV, intersegmental vessel; S, somite.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg standard conditions Prim-15 intersegmental vessel IHC
y1Tg + MO1-thsd7aa standard conditions Prim-15 intersegmental vessel IHC
thsd7aa y1Tg standard conditions Prim-15 central nervous system ISH
y1Tg + MO1-thsd7aa standard conditions Prim-15 central nervous system ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-thsd7aa standard conditions Prim-15 blood vessel endothelial cell migration disrupted, abnormal
Prim-15 intersegmental vessel spatial pattern, abnormal
y1Tg + MO2-thsd7aa standard conditions Prim-15 blood vessel endothelial cell migration disrupted, abnormal
Prim-15 intersegmental vessel spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.