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ZFIN ID: ZDB-FIG-110317-13
Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod. Developmental Biology   350(2):464-475 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Bud to 5-9 somites

Fig. 3 Hh causes Cdkn1c to stabilize Myod. (A). Cdkn1c mRNA is Hh-dependent in adaxial cells. Shhatbx392 or smob641 mutation or cyA treatment reduces cdkn1c mRNA. Note that loss was proportional to reduction in Hh signal (arrowheads and black bracket). Shha mRNA injection caused unilateral somitic cdkn1c up-regulation (red bracket). (B,C). Cdkn1c 5′ MO injection into embryos from a shhatbx392/+ incross reduced adaxial myog mRNA (B), Myod or MyHC proteins (C). Note the delay in myog mRNA and reduction in Myod protein (brackets). (D). Cdkn1c ATG MO injection reduces adaxial Myod at tailbud stage. Bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdkn1ca WT control 10-13 somites adaxial cell ISH
WT standard conditions 5-9 somites adaxial cell ISH
Prim-5 adaxial cell ISH
WT chemical treatment: Cyclopamine 5-9 somites Not Detected adaxial cell ISH
10-13 somites adaxial cell ISH
shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell ISH
smob641/b641 standard conditions Prim-5 adaxial cell ISH
myf5 WT standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
WT + MO3-cdkn1ca standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
shhatbx392/tbx392 standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
shhatbx392/tbx392 + MO3-cdkn1ca standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
myod1 Ab1-myod1 WT control Bud adaxial cell IHC
Ab1-myod1 WT standard conditions 5-9 somites adaxial cell IHC
Ab1-myod1 5-9 somites somite IHC
Ab1-myod1 WT + MO2-cdkn1ca standard conditions Bud adaxial cell IHC
Ab1-myod1 WT + MO3-cdkn1ca standard conditions 5-9 somites adaxial cell IHC
Ab1-myod1 5-9 somites somite IHC
Ab1-myod1 shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell IHC
Ab1-myod1 5-9 somites somite IHC
Ab1-myod1 shhatbx392/tbx392 + MO3-cdkn1ca standard conditions 5-9 somites Not Detected adaxial cell IHC
Ab1-myod1 5-9 somites somite IHC
myog WT standard conditions 5-9 somites adaxial cell ISH
WT + MO3-cdkn1ca standard conditions 5-9 somites adaxial cell ISH
shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell ISH
shhatbx392/tbx392 + MO3-cdkn1ca standard conditions 5-9 somites Not Detected adaxial cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-A4.1025 IHC WT standard conditions 14-19 somites adaxial cell
IHC WT + MO3-cdkn1ca standard conditions 14-19 somites adaxial cell
IHC shhatbx392/tbx392 standard conditions 14-19 somites adaxial cell
IHC shhatbx392/tbx392 + MO3-cdkn1ca standard conditions 14-19 somites adaxial cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-cdkn1ca standard conditions Bud adaxial cell decreased amount, abnormal
WT + MO3-cdkn1ca standard conditions 5-9 somites adaxial cell decreased amount, abnormal
shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell decreased amount, abnormal
shhatbx392/tbx392 + MO3-cdkn1ca standard conditions 5-9 somites adaxial cell absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.