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ZFIN ID: ZDB-FIG-110317-11
Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod. Developmental Biology   350(2):464-475 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 5-9 somites

Fig. 1 Myod protein level correlates with slow myogenesis in Hh signalling mutants. In situ mRNA hybridization for myf5, myod, myog and hsp90a and immunodetection of Myod and Myosin Heavy Chain (MyHC) in dorsal flatmounts of 5 ss stage control, cyclopamine-treated (cyA), smob641 and shhatbx392 mutant embryos. Mutants were identified by significant reduction in one or both markers in ~ 25% of lay (shhatbx392: 9/41; smob641 14/50). Anterior to top. Bar: 100 μm. (A). Absence of MyHC in smob641 but only reduction in shhatbx392 mutants, despite indistinguishable loss of myod and myf5 mRNAs in adaxial cells in anterior pre-somitic mesoderm (arrows). Note that myf5 and myod expression in tailbud (asterisk) and presumptive fast muscle precursors (black arrowheads) is little affected by Hh manipulation. Shhatbx392 differs from smob641 only in expression of myod in differentiating adaxial muscle in somites (white arrowheads). (B). Myog mRNA is present in adaxial cells of shhatbx392 but not smob641 mutants. (C). Myod protein is absent in adaxial cells of cyA and smob641 embryos but weakly present in shhatbx392 mutant. Control embryos were either shhatbx392 siblings or untreated wild type.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hsp90aa1.1 WT standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
smob641/b641 standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
myf5 WT standard conditions 5-9 somites adaxial cell ISH
5-9 somites fast muscle myoblast ISH
5-9 somites segmental plate ISH
5-9 somites tail bud ISH
shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell ISH
5-9 somites fast muscle myoblast ISH
5-9 somites segmental plate ISH
5-9 somites tail bud ISH
smob641/b641 standard conditions 5-9 somites adaxial cell ISH
5-9 somites fast muscle myoblast ISH
5-9 somites segmental plate ISH
5-9 somites tail bud ISH
myod1 Ab1-myod1 WT control 5-9 somites adaxial cell IHC
Ab1-myod1 5-9 somites somite IHC
WT standard conditions 5-9 somites adaxial cell ISH
5-9 somites fast muscle myoblast ISH
5-9 somites tail bud ISH
Ab1-myod1 WT chemical treatment: Cyclopamine 5-9 somites Not Detected adaxial cell IHC
Ab1-myod1 5-9 somites somite IHC
shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell ISH
Ab1-myod1 5-9 somites adaxial cell IHC
5-9 somites fast muscle myoblast ISH
Ab1-myod1 5-9 somites somite IHC
5-9 somites tail bud ISH
Ab1-myod1 smob641/b641 standard conditions 5-9 somites Not Detected adaxial cell IHC
5-9 somites adaxial cell ISH
5-9 somites fast muscle myoblast ISH
Ab1-myod1 5-9 somites somite IHC
5-9 somites tail bud ISH
myog WT standard conditions 5-9 somites adaxial cell ISH
shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell ISH
smob641/b641 standard conditions 5-9 somites Not Detected adaxial cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT standard conditions 5-9 somites adaxial cell
IHC shhatbx392/tbx392 standard conditions 5-9 somites adaxial cell
IHC smob641/b641 standard conditions 5-9 somites Not Detected adaxial cell
Phenotype Details
Fish Conditions Stage Phenotype
shhatbx392/tbx392 standard conditions 5-9 somites segmental plate anterior region has fewer parts of type adaxial cell, abnormal
5-9 somites skeletal muscle tissue development disrupted, abnormal
smob641/b641 standard conditions 5-9 somites segmental plate anterior region lacks all parts of type adaxial cell, abnormal
5-9 somites skeletal muscle tissue development disrupted, abnormal
5-9 somites slow muscle cell aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.