|ZFIN ID: ZDB-FIG-110214-22|
Fig. S1 uhrf1hi3020 is a null allele. (A) Schematic of the uhrf1 genomic locus with the approximate position of the proviral insert and viral or exon-specific primers used for RT-PCR analyses indicated. (B) RT-PCR using the indicated primer sets reveals an absence of uhrf1 transcripts in recessive mutants. Beta actin is used as a loading control. (C) Western blot using protein extracts derived from whole, de-yolked 5 dpf wild-type sibling and uhrf1 mutant embryos. Anti-Uhrf1 recognizes a band of approximately 85 kDa in wild-type samples, which is absent in those from uhrf1 mutants. anti-Hdac1 is used as a loading control.
|uhrf1||Ab1-uhrf1||WT||standard conditions||Day 5||whole organism||WB|
|Ab1-uhrf1||uhrf1hi3020Tg/hi3020Tg||standard conditions||Day 5||Not Detected||whole organism||WB|
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 350(1), Tittle, R.K., Sze, R., Ng, A., Nuckels, R.J., Swartz, M.E., Anderson, R.M., Bosch, J., Stainier, D.Y., Eberhart, J.K., and Gross, J.M., Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens, 50-63, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.