ZFIN ID: ZDB-FIG-110214-22
Tittle et al., 2011 - Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens. Developmental Biology   350(1):50-63 Full text @ Dev. Biol.
Anatomical Term:
Stage: Day 5

Fig. S1 uhrf1hi3020 is a null allele. (A) Schematic of the uhrf1 genomic locus with the approximate position of the proviral insert and viral or exon-specific primers used for RT-PCR analyses indicated. (B) RT-PCR using the indicated primer sets reveals an absence of uhrf1 transcripts in recessive mutants. Beta actin is used as a loading control. (C) Western blot using protein extracts derived from whole, de-yolked 5 dpf wild-type sibling and uhrf1 mutant embryos. Anti-Uhrf1 recognizes a band of approximately 85 kDa in wild-type samples, which is absent in those from uhrf1 mutants. anti-Hdac1 is used as a loading control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
uhrf1 Ab1-uhrf1 WT standard conditions Day 5 whole organism WB
Ab1-uhrf1 uhrf1hi3020Tg/hi3020Tg standard conditions Day 5 Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details No data available
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Reprinted from Developmental Biology, 350(1), Tittle, R.K., Sze, R., Ng, A., Nuckels, R.J., Swartz, M.E., Anderson, R.M., Bosch, J., Stainier, D.Y., Eberhart, J.K., and Gross, J.M., Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens, 50-63, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.