ZFIN ID: ZDB-FIG-110111-19
Feng et al., 2010 - Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation. PLoS Biology   8(12):e1000562 Full text @ PLoS Biol.
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth

Fig. 8 Analysis of transformed cell clones in larvae where immune cell interaction is disrupted.

(A–C) Typical confocal Z-projections of 3-dpf V12RAS+ larvae that were previously injected with (A) control (n = 26), (B) pu.1 (n = 31), and (C) DUOX (n = 14) morpholinos. (D) Quantification of the numbers of V12RAS+ cells in equivalent flank skin within these three groups at 3 dpf. (E and F) Typical confocal Z-projection images of control and DPI-treated larvae at 60 hpf for similar analysis of V12RAS+ cells. (G) Quantification of V12RAS+ cell numbers in control (n = 10) versus DPI-treated (n = 12) larvae at 60 hpf; *, p<0.05; ***, p<0.001. (H) Graphic representation of BrdU incorporation by V12RAS+ cells in pu.1 morphant (n = 33) versus control (n = 21) larvae. Each point on the scatter plots represents one larva. Scale bar = 150 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP io006Tg; zf391Tg control Protruding-mouth motile cell IFL
io006Tg; zf391Tg + MO1-duox standard conditions Protruding-mouth motile cell IFL
io006Tg; zf391Tg + MO1-spi1b standard conditions Protruding-mouth motile cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.