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ZFIN ID: ZDB-FIG-110111-19
Feng et al., 2010 - Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation. PLoS Biology   8(12):e1000562 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth

Fig. 8 Analysis of transformed cell clones in larvae where immune cell interaction is disrupted.

(A–C) Typical confocal Z-projections of 3-dpf V12RAS+ larvae that were previously injected with (A) control (n = 26), (B) pu.1 (n = 31), and (C) DUOX (n = 14) morpholinos. (D) Quantification of the numbers of V12RAS+ cells in equivalent flank skin within these three groups at 3 dpf. (E and F) Typical confocal Z-projection images of control and DPI-treated larvae at 60 hpf for similar analysis of V12RAS+ cells. (G) Quantification of V12RAS+ cell numbers in control (n = 10) versus DPI-treated (n = 12) larvae at 60 hpf; *, p<0.05; ***, p<0.001. (H) Graphic representation of BrdU incorporation by V12RAS+ cells in pu.1 morphant (n = 33) versus control (n = 21) larvae. Each point on the scatter plots represents one larva. Scale bar = 150 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP io006Tg; zf391Tg control Protruding-mouth motile cell IFL
io006Tg; zf391Tg + MO1-duox standard conditions Protruding-mouth motile cell IFL
io006Tg; zf391Tg + MO1-spi1b standard conditions Protruding-mouth motile cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.