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ZFIN ID: ZDB-FIG-110111-13
Feng et al., 2010 - Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation. PLoS Biology   8(12):e1000562 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Day 4

Fig. 2 Comparison of leukocyte recruitment to V12RAS+ transformed cells versus to a wound.

(A) Confocal image of the trunk epidermis (region indicated by schematic) from a movie of a control Tg(kita:GalTA4, UAS:eGFP, LysC:DsRed) larva (Video S3A). (B) DIC image of a skin laser wound in the same region of the trunk as the transformed cells in (C). Inset is a confocal image of the wound highlighting how LysC:DsRed+ cells are recruited to the wound center (Video S3B). (C) Equivalent image to (A) but from a Tg(kita:GalTA4, UAS:V12RASeGFP, LysC:DsRed) larva (Video S3C). (D–F) Superimposition of DsRed+ cell profiles (orange) from all time frames of 80-min time-lapse movies of (D) control, (E) wounded, and (F) V12RAS+ larvae, to reveal cumulative footprints (orange). (G–I) as for (D–F) but with tracks of all the LysC:DsRed+ cells that migrated within the field of view of the movies superimposed onto a single movie still. (J) Quantification of the numbers of LysC:DsRed+ cells that migrate through the imaging fields of control, V12RAS+, and wounded larvae. (K) As for (J) but quantification of the cell MI for each of these conditions. **, p<0.01; ***, p<0.001. Scale bars = 48 μm for all the images. In all images larvae are 4-dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed hzm1Et; io006Tg; nz50Tg standard conditions Day 4 unspecified IFL
EGFP hzm1Et; io006Tg; nz50Tg standard conditions Day 4 unspecified IFL
hzm1Et; nz50Tg; zf390Tg standard conditions Day 4 unspecified IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.