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General Information
ZFIN ID: ZDB-FIG-110111-13
Feng et al., 2010 - Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation. PLoS Biology   8(12):e1000562 Full text @ PLoS Biol.
Anatomical Term:
Stage: Day 4

Fig. 2 Comparison of leukocyte recruitment to V12RAS+ transformed cells versus to a wound.

(A) Confocal image of the trunk epidermis (region indicated by schematic) from a movie of a control Tg(kita:GalTA4, UAS:eGFP, LysC:DsRed) larva (Video S3A). (B) DIC image of a skin laser wound in the same region of the trunk as the transformed cells in (C). Inset is a confocal image of the wound highlighting how LysC:DsRed+ cells are recruited to the wound center (Video S3B). (C) Equivalent image to (A) but from a Tg(kita:GalTA4, UAS:V12RASeGFP, LysC:DsRed) larva (Video S3C). (D–F) Superimposition of DsRed+ cell profiles (orange) from all time frames of 80-min time-lapse movies of (D) control, (E) wounded, and (F) V12RAS+ larvae, to reveal cumulative footprints (orange). (G–I) as for (D–F) but with tracks of all the LysC:DsRed+ cells that migrated within the field of view of the movies superimposed onto a single movie still. (J) Quantification of the numbers of LysC:DsRed+ cells that migrate through the imaging fields of control, V12RAS+, and wounded larvae. (K) As for (J) but quantification of the cell MI for each of these conditions. **, p<0.01; ***, p<0.001. Scale bars = 48 μm for all the images. In all images larvae are 4-dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed hzm1Et; io006Tg; nz50Tg standard conditions Day 4 unspecified IFL
EGFP hzm1Et; io006Tg; nz50Tg standard conditions Day 4 unspecified IFL
hzm1Et; nz50Tg; zf390Tg standard conditions Day 4 unspecified IFL
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.