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ZIRC
ZFIN ID: ZDB-FIG-110105-52
Oehlers et al., 2011 - A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents. Developmental dynamics : an official publication of the American Association of Anatomists   240(1):288-298 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 6

Fig. 4 Intestinal vasculature is disrupted by trinitrobenzene sulfonic acid (TNBS) exposure. A: Comparison of vasculature in live 6 days postfertilization (dpf) TG(fli1a:EGFP)γ1 transgenic larvae imaged under epifluorescence with a GFP filter and (B) representative close up images. Scale bars represent 100 μm. C: Quantification of intestinal bulb vasculature at 6 dpf; mean numbers of subintestinal branches as indicated (n = 45). D: Quantitative polymerase chain reaction analysis of fli1 and vegfa transcription in 6 dpf larvae exposed to TNBS, shown relative to controls (RQ = 1; n = 10, pools of 20 larvae). All error bars represent a 95% confidence interval of the mean, P values calculated by t-test. **P < 0.01, ***P < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Day 6 intestine vasculature IFL
y1Tg chemical treatment: pharmaceutical Day 6 intestine vasculature IFL
fli1a y1Tg control Day 6 whole organism RTPCR
y1Tg chemical treatment: pharmaceutical Day 6 whole organism RTPCR
vegfab y1Tg control Day 6 whole organism RTPCR
y1Tg chemical treatment: pharmaceutical Day 6 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg chemical treatment: pharmaceutical Day 6 intestine vasculature branchiness, abnormal
Day 6 intestine vasculature structure, normal
Day 6 trunk vasculature normal amount, normal
Day 6 trunk vasculature spatial pattern, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.