ZFIN ID: ZDB-FIG-101206-45
Choy et al., 2010 - A cascade of irx1a and irx2a controls shh expression during retinogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   239(12):3204-3214 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 3 irx2a is required for neurogenesis of the retina ganglion cells. A–L: Immunostaining of 72-hpf retina. A–I: Lamination defects of irx2a MO2 morphant retinas were revealed by H&E staining. In the control morphant (A), the retina was segregated into three distinct nuclear layers (outer nuclear layer [ONL], inner nuclear layer [INL, and ganglion cell layer [GCL]). In contrast, the laminar structure was disrupted in the irx2a MO2 morphant retina (E). HuC/D antibody was used to label differentiated RGCs in the GCL (B). However, HuC/D staining was barely detectable in the morphant retina (F). Zn8 immunoreactivity was observed in the surface of RGC and axons in the optic nerve (C). Zn8 staining was completely absent in the morphant (G). Zn12 immunostaining labeled RGCs and amacrine cells in the INL of the control retina (D), but only a small patch of staining was detected in the morphant retina (H). Neurogenesis defects in the GCL and INL of the morphant retina were rescued by injection of irx2a mRNA (I–L). M: Co-injection of irx2a mRNA, but not irx1a, irx4a, rescued the irx2a morphant phenotype.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-elavl IHC WT control Protruding-mouth retinal ganglion cell
IHC WT + MO2-irx2a standard conditions Protruding-mouth retina
zn-8 IHC WT control Protruding-mouth cranial nerve II
IHC Protruding-mouth retinal ganglion cell
IHC WT + MO2-irx2a standard conditions Protruding-mouth Not Detected retina
zn-12 IHC WT control Protruding-mouth amacrine cell
IHC Protruding-mouth retinal ganglion cell
IHC WT + MO2-irx2a standard conditions Protruding-mouth retina
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-irx2a standard conditions Protruding-mouth retina lacks parts or has fewer parts of type retinal inner plexiform layer, abnormal
Protruding-mouth retina lacks parts or has fewer parts of type retinal outer plexiform layer, abnormal
Protruding-mouth retina layer formation disrupted, abnormal
Protruding-mouth retinal ganglion cell layer hypoplastic, abnormal
Protruding-mouth retinal inner nuclear layer hypoplastic, abnormal
Protruding-mouth whole organism lacks all parts of type cranial nerve II, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.