ZFIN ID: ZDB-FIG-101111-51
Nourizadeh-Lillabadi et al., 2010 - Early embryonic gene expression profiling of zebrafish prion protein (prp2) morphants. PLoS One   5(10):e13573 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 3 Neuronal structures were visualized by the Zn12 antibody (Yellow), whereas Prp2 was detected with a rabbit anti serum (Red). A) In the wild type 24 hpf Prp2 is observed in the trigeminal ganglion and telencephalon, but also present in other neuronal tissue. B) Neuronal structures visualized by HNK-1 staining (Zn-12 antibody) in the same specimen as in A. C) Aberrant morphology of the trigeminal ganglion in Prp2 morphants (arrow) as well as reduced number of peripheral neurons visualized by HNK-1 staining. D) Non deconvolved single plane image of a morphant with condensed nuclei (inside stippled ring). Abbreviations: eye (e), telencephalon (t) and trigeminal ganglion (tg).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
prnprs3 WT standard conditions Prim-5 nervous system IHC
Prim-5 telencephalon IHC
Prim-5 trigeminal ganglion IHC
WT + MO4-prnprs3 standard conditions Prim-5 Not Detected nervous system IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-12 IHC WT standard conditions Prim-5 nervous system neuron projection
IHC Prim-5 telencephalon
IHC Prim-5 trigeminal ganglion
IHC WT + MO4-prnprs3 standard conditions Prim-5 nervous system neuron projection
IHC Prim-5 trigeminal ganglion
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-prnprs3 standard conditions Prim-5 eye apoptotic, abnormal
Prim-5 head peripheral neuron decreased amount, abnormal
Prim-5 hindbrain apoptotic, abnormal
Prim-5 hindbrain morphology, abnormal
Prim-5 midbrain apoptotic, abnormal
Prim-5 midbrain morphology, abnormal
Prim-5 olfactory placode apoptotic, abnormal
Prim-5 trigeminal ganglion apoptotic, abnormal
Prim-5 trigeminal ganglion morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One