ZFIN ID: ZDB-FIG-101105-37
Jurynec et al., 2010 - SHIP2, a factor associated with diet-induced obesity and insulin sensitivity, attenuates FGF signaling in vivo. Disease models & mechanisms   3(11-12):733-742 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Sphere to 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Sphere to Prim-5

Fig. 2 Knockdown of maternal Ship2a dorsalizes the embryo and affects patterning prior to the onset of gastrulation. (A-F) Lateral expansion of dorsal tissue fates in 5–6-somite stage embryos depleted for Ship2a protein. Maternal ship2a-morphant embryos have a lateral expansion of somites (A,B) and a lateral expansion of krox-20 expression in rhombomeres 3 and 5 (r3 and r5) (C,D) compared with WT controls. (E,F) foxd3 expression at the lateral border of the neural plate is reduced in maternal ship2a-morphant embryos. (G,H) ship2a morphants that survive to 24 hpf appear dorsalized. (I–N) foxb1.2 and eve1 expression at the sphere stage (4 hpf) in WT and maternal ship2a-morphant embryos. (I,K) foxb1.2 is expressed dorsally in WT embryos, and (J,L) its expression is expanded to the ventral side of the embryo in maternal ship2a morphants. (M) eve1 expression is restricted to the ventral side in WT embryos, and (N) ventral eve1 expression is lost in maternal ship2a morphants. (O,P) goosecoid (gsc) expression marking the dorsal organizer at 70% epiboly is unchanged between WT and maternal ship2a-morphant embryos. A and B, dorsal views, anterior to the left; C-F, dorsal views, anterior to top; G and H, lateral views; I-N, animal pole views with dorsal to the right; O and P, lateral views, dorsal to right, markings indicate blastoderm margin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
eve1 AB standard conditions Sphere blastodisc ventral region ISH
AB + MO1-inppl1a standard conditions Sphere Not Detected blastodisc ISH
foxb1a AB standard conditions Sphere blastodisc dorsal region ISH
5-9 somites neural plate lateral margin ISH
AB + MO1-inppl1a standard conditions Sphere blastodisc ISH
5-9 somites neural plate lateral margin ISH
AB + MO2-inppl1a standard conditions Sphere blastodisc ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-inppl1a standard conditions Sphere whole organism wholly dorsalized, abnormal
Shield shield morphology, normal
5-9 somites rhombomere 3 increased width, abnormal
5-9 somites rhombomere 5 increased width, abnormal
5-9 somites somite decreased length, abnormal
5-9 somites somite increased width, abnormal
Prim-5 whole organism wholly dorsalized, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.