ZFIN ID: ZDB-FIG-101025-20
Wong et al., 2010 - Duplicate dmbx1 genes regulate progenitor cell cycle and differentiation during zebrafish midbrain and retinal development. BMC Developmental Biology   10:100 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 6 Altered retinal gene expression in morphant embryos. Coronal plastic sections (~ 1 μm) of retina at 72 hpf after whole mount in situ hybridization labeling for progenitor markers from control mMO1a + mMO1b injected (A, C, E), or MO1a + MO1b injected (B, D, F) embryos. Position of the lens (shown in A) is similar for all panels. Arrows in C and D demarcate the ciliary marginal zone (CMZ). Cryosections (~15 μm) of control (G, I, K, M, O, Q) and MO1a + MO1b (H, J, L, N, P, R) embryos immunolabelled with antibodies for indicated differentiation markers (red or green) and counter-stained with DAPI (blue). MO, morpholino.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
neurod1 AB standard conditions Protruding-mouth eye photoreceptor cell ISH
AB + MO1-dmbx1a + MO1-dmbx1b standard conditions Protruding-mouth eye photoreceptor cell ISH
otx2b AB standard conditions Protruding-mouth Not Detected ciliary marginal zone ISH
Protruding-mouth Not Detected retinal ganglion cell layer ISH
Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth Not Detected retinal outer nuclear layer ISH
AB + MO1-dmbx1a + MO1-dmbx1b standard conditions Protruding-mouth retinal ganglion cell layer ISH
Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth retinal outer nuclear layer ISH
vsx2 AB standard conditions Protruding-mouth ciliary marginal zone somatic stem cell ISH
AB + MO1-dmbx1a + MO1-dmbx1b standard conditions Protruding-mouth ciliary marginal zone somatic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zc7Tg + MO1-dmbx1a + MO1-dmbx1b standard conditions Protruding-mouth amacrine cell decreased amount, abnormal
Protruding-mouth retina neuronal stem cell undifferentiated, abnormal
Protruding-mouth retinal bipolar neuron decreased amount, abnormal
Protruding-mouth retinal ganglion cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.