ZFIN ID: ZDB-FIG-101021-56
Fukazawa et al., 2010 - poky/chuk/ikk1 is required for differentiation of the zebrafish embryonic epidermis. Developmental Biology   346(2):272-283 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Sphere to 50%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Sphere to 50%-epiboly

Fig. 3  poky mutant embryos display junction protein localization and apical–basal polarity. Wild type embryos displayed tight localization of actin microfilaments at cell–cell boundaries (arrows) at sphere (A, magnified in C) and robust labeling of cell vertices at 50% epiboly (E magnified in G, arrowheads). poky mutant embryos displayed actin localization to cell–cell boundaries (arrows) at sphere stage (B magnified in D). At 50% epiboly (F magnified in H) the localization to cell–cell boundaries was less robust. Gaps were observed between neighboring cells (arrows) and little labeling was observed at cell vertices (arrowheads). Wild type embryos displayed localization of ZO1 to the tight junction at EVL cell borders at sphere (I magnified in K) and 50% epiboly (M magnified in O) with strong localization to cell–cell boundaries (arrows) and cell vertices (arrowheads). poky mutants also displayed localization at sphere stage (J, magnified in L). By 50% epiboly poky mutant cells were smaller but still displayed localized ZO1 (N magnified in P, arrow), although they lacked cell vertex labeling (arrowhead, N,P). Wild type (Q,S,U) and poky mutant (R,T,V) embryos display apical localization of aPKC (green, Q–T), localization of ZO1 to sites of EVL cell–cell contact (red, Q,R), basolateral localization of β-catenin (red, S,T) and cadherin (U,V). (W–Z) TEM of wild type (W,X) and poky mutant (Y,Z) EVL cells at 30% epiboly (tight junctions, black arrows).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prkcaa Ab1-prkca WT standard conditions 50%-epiboly EVL apical part of cell IHC
Ab1-prkca chukp20ad/p20ad standard conditions 50%-epiboly EVL apical part of cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pan-Cadherin IHC WT standard conditions 50%-epiboly EVL basal part of cell
IHC chukp20ad/p20ad standard conditions 50%-epiboly EVL basal part of cell
Ab1-tjp1 IHC WT standard conditions Sphere superficial blastomere bicellular tight junction
IHC 50%-epiboly EVL bicellular tight junction
IHC chukp20ad/p20ad standard conditions Sphere superficial blastomere bicellular tight junction
IHC 50%-epiboly EVL bicellular tight junction
Ab3-ctnnb IHC WT standard conditions 50%-epiboly EVL basal part of cell
IHC chukp20ad/p20ad standard conditions 50%-epiboly EVL basal part of cell
Phenotype Details
Fish Conditions Stage Phenotype
chukp20ad/p20ad standard conditions Sphere EVL actin filament disorganized, abnormal
chukp20ad/p20ad standard conditions 50%-epiboly EVL actin filament disorganized, abnormal
chukp20ad/p20ad standard conditions 50%-epiboly EVL bicellular tight junction irregular spatial pattern, abnormal
chukp20ad/p20ad standard conditions 50%-epiboly EVL cell apical-basal polarity, normal
chukp20ad/p20ad standard conditions 50%-epiboly EVL cell junction immature, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 346(2), Fukazawa, C., Santiago, C., Park, K.M., Deery, W.J., Canny, S.G., Holterhoff, C.K., and Wagner, D.S., poky/chuk/ikk1 is required for differentiation of the zebrafish embryonic epidermis, 272-283, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.