ZFIN ID: ZDB-FIG-101013-1
Van Otterloo et al., 2010 - Differentiation of zebrafish melanophores depends on transcription factors AP2 alpha and AP2 epsilon. PLoS Genetics   6(9):pii: e1001122 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 5 tfap2a/e doubly-deficient embryos have defects in melanophore differentiation.

(A–L) Lateral views of 29 hpf embryos processed to reveal (A–D) dct, (E–H) tyrp1b, and (I–L) tyr expression. (A, E, I) Sibling embryos have greater numbers of cells expressing these markers than do (C, G, K) tfap2a mutants; (D, H, L) in tfap2a-/-/eMO embryos a further reduction is apparent. This enhanced reduction is most apparent for dct expression. (B, F, J) Sibling embryos injected with the tfap2e MO resemble uninjected sibling embryos. Scale bars: 100 μm. (M) Histogram showing average number of dct-positive, tyrp1b-positive, and tyr-positive cells in the whole embryo at 29 hpf. First pair of bars, dct-positive cells in uninjected tfap2a-/- embryos (n = 10 embryos), vs. in tfap2a-/-/eMO embryos (n = 20 embryos); second pair of bars, tyrp1b-positive cells in the uninjected tfap2a-/- embryos (n = 10 embryos) vs. in tfap2a-/-/eMO embryos (n = 20 embryos). Final pair of bars, tyr-positive cells in uninjected tfap2-/- embryos (n = 10 embryos) vs. in tfap2a-/-/eMO embryos (n = 10 embryos). Student t-test analyses indicate that the differences among cells expressing the indicated markers are statistically significant for tfap2a-deficient embryos vs. tfap2a/e-deficient embryos (for dct, p = 1.4×10-9; for tyrp1b, p = 1.5×10-8; for tyr, p = 0.02). (N) mRNA expression levels of differentiation markers in cells sorted from mitf:egfp embryos injected with the tfap2a/e MO (normalized to β-actin) relative to those in cells sorted from embryos injected with the tfap2a MO alone (normalized to β-actin) (* = p<0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dct WT control Prim-5 melanocyte ISH
WT + MO2-tfap2e + MO4-tp53 standard conditions Prim-5 melanocyte ISH
tfap2ats213/ts213 control Prim-5 melanocyte ISH
tfap2ats213/ts213 + MO2-tfap2e + MO4-tp53 standard conditions Prim-5 melanocyte ISH
tyr WT control Prim-5 melanocyte ISH
WT + MO2-tfap2e + MO4-tp53 standard conditions Prim-5 melanocyte ISH
tfap2ats213/ts213 control Prim-5 melanocyte ISH
tfap2ats213/ts213 + MO2-tfap2e + MO4-tp53 standard conditions Prim-5 melanocyte ISH
tyrp1b WT control Prim-5 melanocyte ISH
WT + MO2-tfap2e + MO4-tp53 standard conditions Prim-5 melanocyte ISH
tfap2ats213/ts213 control Prim-5 melanocyte ISH
tfap2ats213/ts213 + MO2-tfap2e + MO4-tp53 standard conditions Prim-5 melanocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tfap2ats213/ts213 control Prim-5 melanocyte decreased amount, abnormal
Prim-5 melanocyte differentiation disrupted, abnormal
tfap2ats213/ts213 + MO2-tfap2e + MO4-tp53 standard conditions Prim-5 melanocyte decreased amount, abnormal
Prim-5 melanocyte differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.