ZFIN ID: ZDB-FIG-100518-9
Heisenberg et al., 1996 - Genes involved in forebrain development in the zebrafish, Danio rerio. Development (Cambridge, England)   123:191-203
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 3

Whole-mount antibody stainings of embryos for pax6 (30 hour), islet proteins (antipan- islet antibody; 24 hour), and fret43 (24 hour) visualizing the anterior pituitary and epiphysis in wild-type (A-C) and mbl (D-F) embryos. (A,D) Ventral view of the head of embryos stained for pax6 showing a reduction in the number of pax6-positive neurons within the anterior pituitary (arrowhead) of mbl embryos. (B,C,E,F) Dorsal view of the head of embryos stained for islet proteins (B,E) and fret43 (C,F) showing an increase in the number of islet/fret43-positive cells within the epiphysis (arrowhead) of mbl. Anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax6a WT standard conditions Prim-15 adenohypophyseal placode neuron IHC
axin1tm13/tm13 standard conditions Prim-15 adenohypophyseal placode neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-isl IHC WT standard conditions Prim-5 epiphysis
IHC axin1tm13/tm13 standard conditions Prim-5 epiphysis
zpr-1 IHC WT standard conditions Prim-5 epiphysis
IHC axin1tm13/tm13 standard conditions Prim-5 epiphysis
Phenotype Details
Fish Conditions Stage Phenotype
axin1tm13/tm13 standard conditions Prim-5 epiphysis increased size, abnormal
axin1tm13/tm13 standard conditions Prim-15 adenohypophysis decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.