ZFIN ID: ZDB-FIG-100504-13
Tay et al., 2010 - A vertebrate-specific Chp-PAK-PIX pathway maintains E-cadherin at adherens junctions during zebrafish epiboly. PLoS One   5(4):e10125 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Long-pec

Fig. 1 Protein sequence analysis and spatio-temporal expression of Chp.

(A) The relationship between Chp, Cdc42 and Rac in zebrafish (Danio rerio). Protein sequences were aligned using ClustalW (DNAStar) and the dendrogram generated by Phylip. Accession numbers for cDNA sequences of Cdc42 and Rac family are: Rac1a, AY865568. Rac1b, XP_001332092.1. Rac2, AY865569. Rac3, AY865570. Cdc42a, AY865566. Cdc42b, XM_678979. Cdc42c, AY865567. Rhoua, AY865564. Rhoub, NM_001017784, Chp/ RhoV, NM_001012250. (B) RT-PCR products showing mRNA transcript profile of zebrafish Chp at different developmental stages as indicated. Both Cdc42a and Cdc42c were detected throughout the stages tested. Chp transcripts appear at epiboly. (C) Whole mount in situ hybridization (WISH) using dioxygenin (DIG) anti-sense Chp RNA probes indicates all cell types express the transcript at 50% and 80% epiboly (lateral views): Chp mRNA is enriched in notochord (indicated by arrows) at 24 hpf and 48 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdc42 WT standard conditions 1-cell whole organism RTPCR
128-cell whole organism RTPCR
50%-epiboly whole organism RTPCR
30%-epiboly whole organism RTPCR
75%-epiboly whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
cdc42l WT standard conditions 1-cell whole organism RTPCR
128-cell whole organism RTPCR
30%-epiboly whole organism RTPCR
50%-epiboly whole organism RTPCR
75%-epiboly whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
cdc42l2 WT standard conditions 1-cell Not Detected whole organism RTPCR
128-cell Not Detected whole organism RTPCR
50%-epiboly Not Detected whole organism RTPCR
30%-epiboly Not Detected whole organism RTPCR
75%-epiboly Not Detected whole organism RTPCR
10-13 somites Not Detected whole organism RTPCR
Prim-5 Not Detected whole organism RTPCR
Long-pec Not Detected whole organism RTPCR
rhov WT standard conditions 1-cell Not Detected whole organism RTPCR
128-cell Not Detected whole organism RTPCR
50%-epiboly whole organism RTPCR
30%-epiboly whole organism RTPCR
Shield whole organism ISH
50%-epiboly whole organism ISH
75%-epiboly whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 notochord ISH
Prim-5 whole organism RTPCR
Long-pec notochord ISH
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One